NDLI: Evaluation of three molecular methods of repetitive element loci for differentiation of Mycobacterium avium subsp. paratuberculosis (MAP)

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Journal of Microbiology : Volume 47, Issue 2, April 2009

Journal of Microbiology : Volume 47, Issue 1, February 2009

Journal of Microbiology : Volume 46

Evaluation of three molecular methods of repetitive element loci for differentiation of Mycobacterium avium subsp. paratuberculosis (MAP)

Content Provider	Springer Nature Link
Author	El Sayed, Amr Hassan, Abdulwahed Ahmed Natour, Saleh Abdulmawjood, Amir Bülte, Michael Wolter, Wilfried Zschöck, Michael
Copyright Year	2009
Abstract	The aim of the present study is to evaluate the efficiency of three methods to determine the molecular diversity of 34 Mycobacterium avium subsp. paratuberculosis (MAP) strains isolated from 17 cattle herds. The applied methods included the analysis of sequence polymorphism of the mononucleotide (G1 and G2) and trinucleotide sequences (GGT) of the Short Sequence Repeats (SSR) and the determination of size polymorphism of 9 different Mycobacterial Interspersed Repetitive Units (MIRU) and 6 Variable Number Tandem Repeats (VNTR). Sequence analysis of SSR of 34 isolates showed 4, 6, and 2 alleles of G1, G2, and GGT repeats, respectively. The amplification of the investigated 9 MIRU units revealed only two discriminatory genotyping systems (MIRU2 and MIRU3). Out of 6 VNTR PCR differentiation methods, only one method could be recommended for genotyping purposes. The profile 7g-12g-4ggt-II-b-2 of the combination systems G1-G2-GGT-MIRU2-MIRU3-VNTR1658 dominates among the examined isolates and was detected in 14.7% of the isolates. The use of certain repetitive loci of SSR, MIRU, and VNTR techniques in this study showed greater potential than others for the characterization of MAP isolates. The recommended loci can be used for the epidemiological tracing of MAP field strains and to determine the relationships between isolates in different herds.
Starting Page	253
Ending Page	259
Page Count	7
File Format	PDF
ISSN	12258873
Journal	Journal of Microbiology
Volume Number	47
Issue Number	3
e-ISSN	19763794
Language	English
Publisher	The Microbiological Society of Korea
Publisher Date	2009-06-26
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mycobacterium avium subsp. paratuberculosis (MAP) short sequence repeats (SSR) mycobacterial interspersed repetitive units (MIRU) variable number tandem repeats (VNTR) Johne’s disease (JD) Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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