NDLI: Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis

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Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis

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Journal of Microbiology : Volume 47, Issue 2, April 2009

Journal of Microbiology : Volume 47, Issue 1, February 2009

Journal of Microbiology : Volume 46

Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis

Content Provider	Springer Nature Link
Author	Zhang, Feiwei Qiao, Dairong Xu, Hui Liao, Chong Li, Shilin Cao, Yi
Copyright Year	2009
Abstract	Xylose reductase (XR) is a key enzyme in xylose metabolism because it catalyzes the reduction of xylose to xylitol. In order to study the characteristics of XR from Candida tropicalis SCTCC 300249, its XR gene (xyll) was cloned and expressed in Escherichia coli BL21 (DE3). The fusion protein was purified effectively by Ni$^{2+}$-chelating chromatography, and the kinetics of the recombinant XR was investigated. The Km values of the C. tropicalis XR for NADPH and NADH were 45.5 μM and 161.9 μM, respectively, which demonstrated that this XR had dual coenzyme specificity. Moreover, this XR showed the highest catalytic efficiency (kcat=1.44×l0$^{4}$ min$^{−1}$) for xylose among the characterized aldose reductases. Batch fermentation was performed with Saccharomyces serivisiae W303-lA:pYES2XR, and resulted in 7.63 g/L cell mass, 93.67 g/L xylitol, and 2.34 g/L · h xylitol productivity. This XR coupled with its dual coenzyme specificity, high activity, and catalytic efficiency proved its utility in in vitro xylitol production.
Starting Page	351
Ending Page	357
Page Count	7
File Format	PDF
ISSN	12258873
Journal	Journal of Microbiology
Volume Number	47
Issue Number	3
e-ISSN	19763794
Language	English
Publisher	The Microbiological Society of Korea
Publisher Date	2009-06-26
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Candida tropicalis xylose reductase coenzyme specificity catalytic efficiency Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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