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| Content Provider | Springer Nature Link |
|---|---|
| Author | Chen, Jen Tao Chao, Mei Li Wen, Chiou Yen Chu, Wen Shen |
| Copyright Year | 2012 |
| Abstract | Culture filtrates of 22 mushrooms were screened for extracellular prolyl oligopeptidase activity. Four strains with relatively high enzyme activity were all from inky cap mushrooms. The production of Coprinopsis clastophylla prolyl oligopeptidase was associated with the growth of the fungus and the enzyme was not released by cell lysis. The enzyme was purified 285-fold to a specific activity of 52.05 U/mg. It was purified to a single band on a native polyacrylamide gel. However, the enzyme separated into three bands on a sodium dodecyl sulfate-polyacrylamide gel with mobility corresponding to molecular weights of approximately 84, 60, and 26 kDa. The results of tandem mass spectrometric analysis revealed that the 60 kDa protein was likely a degradation product of the 84 kDa protein. The isoelectric point of the purified enzyme was 5.2. The purified enzyme had an optimal pH and temperature of 8.0 and 37°C, respectively. Diisopropyl fluorophosphate (DFP), p-chloromercuribenzoaic acid (PCMB), Hg$^{2+}$, and Cu$^{2+}$ strongly inhibited C. clastophylla prolyl oligopeptidase. This enzyme is a serine peptidase and one or more cysteine residues of the enzyme are close to the active site. |
| Starting Page | 652 |
| Ending Page | 659 |
| Page Count | 8 |
| File Format | |
| ISSN | 12258873 |
| Journal | Journal of Microbiology |
| Volume Number | 50 |
| Issue Number | 4 |
| e-ISSN | 19763794 |
| Language | English |
| Publisher | The Microbiological Society of Korea |
| Publisher Date | 2012-08-25 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | C. clastophylla inky cap mushrooms extracellular prolyl oligopeptidase serine peptidase purification Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Applied Microbiology and Biotechnology |
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