NDLI: Biocompatibility and internalization of molecularly imprinted nanoparticles

Content Provider	Springer Nature Link
Author	Canfarotta, Francesco Waters, Alicia Sadler, Robyn McGill, Paul Guerreiro, Antonio Papkovsky, Dmitri Haupt, Karsten Piletsky, Sergey
Copyright Year	2016
Abstract	Molecularly imprinted polymers (MIP) are receiving increasing attention thanks to their robustness, stability, and inexpensive manufacture compared with their bio-analogues such as antibodies. The molecular imprinting process can be defined as the generation of molecular recognition sites in a synthetic polymer. The template-derived sites created within a polymeric matrix allow MIPs (often referred as plastic antibodies) to selectively recognize and bind to the target molecule. Therefore, MIPs can be used in sensors and in separation and diagnostics. Owing to their size and functional properties, MIP nanoparticles (NPs) can potentially be used in biomedicine, but comprehensive analysis of their interaction with cells and in vitro toxicological tests must be performed first. Herein, we report the synthesis of bare and core–shell imprinted NPs using an innovative solid-phase approach and the toxicological evaluation of such NPs in different cell lines (HaCaT, MEFs, HT1080, and macrophages). We also evaluated the influence of the protein corona on particle stability, the internalization of NPs in cells, and the influence of various surface coatings. Studies on the metabolic effects of imprinted NPs on fibroblasts showed that bare MIPs do not alter cell metabolism, whereas some issues arise when specific particle coatings are used. Furthermore, in vitro cytokine release studies revealed that macrophages were not activated in the presence of the MIPs evaluated in this study. The results suggest that MIP NPs are biocompatible, paving the way for their in vivo application.
Starting Page	3463
Ending Page	3477
Page Count	15
File Format	PDF
ISSN	19980124
Journal	Nano Research
Volume Number	9
Issue Number	11
e-ISSN	19980000
Language	English
Publisher	Tsinghua University Press
Publisher Date	2016-09-13
Publisher Place	Beijing
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	pegylation core–shell toxicology inflammatory response particle uptake metabolism Nanotechnology Materials Science Atomic/Molecular Structure and Spectra Condensed Matter Physics Biotechnology Biomedicine
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Atomic and Molecular Physics, and Optics Condensed Matter Physics Electrical and Electronic Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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