Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Su, Ya Ru Wang, Jian Wu, Jian Jun Chen, Yan Jiang, Yu Ping |
| Copyright Year | 2007 |
| Abstract | To construct recombinant lentiviral vectors for gene delivery of the glial cell line-derived neurotrophic factor (GDNF), and evaluate the neuroprotective effect of GDNF on lactacystin-damaged PC12 cells by transfecting it into bone marrow stromal cells (BMSCs).PLenti6/V5-GDNF plasmid was set up by double restriction enzyme digestion and ligation, and then the plasmid was transformed into Top10 cells. Purified pLenti6/V5-GDNF plasmids from the positive clones and the packaging mixture were cotransfected to the 293FT packaging cell line by Lipofectamine2000 to produce lentivirus, then the concentrated virus was transduced to BMSCs. Overexpression of GDNF in BMSCs was tested by RT-PCR, ELISA and immunocytochemistry, and its neuroprotection for lactacystin-damaged PC12 cells was evaluated by MTT assay.Virus stock of GDNF was harvested with the titer of 5.6×10$^{5}$ TU/mL. After transduction, GDNF-BMSCs successfully secreted GDNF to supernatant with higher concentration (800 pg/mL) than BMSCs did (less than 100 pg/mL). The supernatant of GDNF-BMSCs could significantly alleviate the damage of PC12 cells induced by lactacystin (10 μmol/L).Overexpression of lentivirus-mediated GDNF in the BMSCs cells can effectively protect PC12 cells from the injury by the proteasome inhibitor.建立慢病毒介导的胶质细胞系源性神经营养因子(glial cell line-derived neurotrophic factor, GDNF)表达系统, 体外感染骨髓基质细胞, 检测过表达GDNF对蛋白酶抑制剂引起的PC12细胞损伤的神经保护作用。经双酶切和T4连接酶构建pLenti6/V5-GDNF表达质粒, 经293FT细胞包装产生高滴度病毒。 用RT-PCR、 ELISA和免疫细胞化学方法检测感染骨髓基质细胞(bone marrow stromal cells, BMSCs)后GDNF的表达, 并检测过表达GDNF对蛋白酶抑制剂乳胞素(lactacystin)引起的PC12细胞损伤的保护作用。成功构建pLenti6/V5-GDNF表达质粒, 获得高滴度具有感染能力的病毒储存液(5.6 × 10$^{5}$ TU/mL)。 BMSCs体外被感染后能大量分泌GDNF(接近800 pg/mL), 过表达GDNF能减轻乳胞素(10 μmol/L)引起的PC12细胞损伤。慢病毒介导的GDNF转染骨髓基质细胞后能分泌具有生物学活性的GDNF, 对蛋白酶体抑制剂引起的PC12细胞损伤有保护作用。 |
| Starting Page | 67 |
| Ending Page | 74 |
| Page Count | 8 |
| File Format | |
| ISSN | 16737067 |
| Journal | Neuroscience Bulletin |
| Volume Number | 23 |
| Issue Number | 2 |
| e-ISSN | 19958218 |
| Language | Chinese |
| Publisher | Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences |
| Publisher Date | 2008-02-01 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Parkinson’s disease proteasome inhibitor glial cell line-derived neurotrophic factor lentivirus gene therapy bone marrow stromal cells Pain Medicine Neurology Anatomy Anesthesiology Human Physiology Neurosciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience Physiology Medicine |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|