NDLI: Morphological and behavioral consequences of recurrent seizures in neonatal rats are associated with glucocorticoid levels

Please wait, while we are loading the content...

Overexpression of lentivirus-mediated glial cell line-derived neurotrophic factor in bone marrow stromal cells and its neuroprotection for the PC12 cells damaged by lactacystin

Expression of motilin in the hypothalamus and the effect of central erythromycin on gastric motility in diabetic rats

Morphological and behavioral consequences of recurrent seizures in neonatal rats are associated with glucocorticoid levels

Facial pain induces the alteration of transient receptor potential vanilloid receptor 1 expression in rat trigeminal ganglion

Changes of Src-suppressed C kinase substrate expression in cytokine induced reactive C6 glioma cells

N-methyl-D-aspartate receptors mediate diphosphorylation of extracellular signal-regulated kinases through Src family tyrosine kinases and Ca$^{2+}$/calmodulin-dependent protein kinase II in rat hippocampus after cerebral ischemia

An intracellular study of pretectal influence on the optic tectum of the frog, Rana catesbeiana

Inhibition of the MAPK/ERK cascade: a potential transcription-dependent mechanism for the amnesic effect of anesthetic propofol

Pathogenesis of Parkinson’s disease: oxidative stress, environmental impact factors and inflammatory processes

Neuroscience Bulletin : Volume 23, Issue 1, January 2007

Morphological and behavioral consequences of recurrent seizures in neonatal rats are associated with glucocorticoid levels

Content Provider	Springer Nature Link
Author	Shi, Xiu Yu Wang, Ji Wen Lei, Ge Fei Sun, Ruo Peng
Copyright Year	2007
Abstract	It is well documented that epilepsy can increase neurogenesis in certain brain regions and cause behavioral alternations in patients and different epileptic animal models. A series of experimental studies have demonstrated that neurogenesis is regulated by various factors including glucocorticoid (CORT), which can reduce neurogenesis. Most of studies in animal have been focused on adulthood stage, while the effect of recurrent seizures to immature brain in neonatal period has not been well established. This study was designed to investigate how the recurrent seizures occurred in the neonatal period affected the immature brain and how CORT regulated neurogenesis in immature animals.Neonatal rats were subjected to 3 pilocarpine-induced seizures from postnatal day 1 to day 7. Then neurogenesis at different postnatal ages (i.e. P8, P12, P22, P50) was observed. Behavioral performance was tested when the rats were mature (P40), and plasma CORT levels following recurrent seizures were simultaneously monitored.Rats with neonatal seizures had a significant reduction in the number of Bromodeoxyuridine (BrdU) labeled cells in the dentate gyrus compared with the control groups when the animals were euthanized on P8 or P12 (P < 0.05); whereas there was no difference between the two groups on P22. Until P50, rats with neonatal seizures had increased number of BrdU-labeled cells compared with the control group (P < 0.05). In Morris water maze task, pilocarpine-treated rats were significantly slower than the control rats at the first and second day, and there were no differences at other days. In probe trial, there was no significant difference in time spent in the goal quadrant between the two groups. Endocrine studies showed a correlation between the number of BrdU positive cells and the CORT level. Sustained increase in circulating CORT levels was observed following neonatal seizures on P8 and P12.Neonatal recurrent seizures can biphasely modulate neurogenesis over different time windows with a down-regulation at early time and up-regulation afterwards, cause persistent deficits in cognitive functions of adults, and increase the circulating CORT levels. CORT levels are related with the morphological and behavioral consequences of recurrent seizures.探讨新生期大鼠反复痫性发作后的形态学, 行为学以及糖皮质激素水平的变化。64只出生后一天的 Wistar 大鼠随机分为惊厥组40只和对照组24只。惊厥组的新生鼠在出生后1天(P1)、 4天(P4)、 7天(P7)给予腹腔注射匹罗卡品, 制备新生鼠癫痫模型, 对照组的新生鼠腹腔注射生理盐水。惊厥组分别在第3次致痫后在即刻(I组)、第4天(I 组)、第 14 天(III组)、第42天(IV组)四个时间点处死, 各时间点设相应对照组, 处死前36 h惊厥组和对照组的大鼠腹腔注射BrdU。所有大鼠处死前均取血检测糖皮质激素。第IV组从P40开始进行Morris水迷宫试验。新生鼠3次发作后即刻和第4天与相应日龄对照组相比, 状回BrdU阳性细胞数明显减少(P < 0.05), 而癫痫发作后14天和42天BrdU阳性细胞数增加, 但发作后14天差异无统计学意义(P > 0.05)。在4天的Morris水迷宫试验中, 匹罗卡品处理组大鼠到达平台的时间均长于对照组, 但是只有第1天和第2天有统计学意义(P < 0.05)。检测结果表明高水平的糖皮质激素一直持续到发作后第4天, 糖皮质激素水平与BrdU阳性细胞数呈负相关。新生大鼠反复痫性发作会造成早期神经发生减少, 而后期神经发生增加; 造成大鼠成年后认知功能缺陷; 造成糖皮质激素水平增高, 这与痫性大鼠形态学和行为学方面的改变有关。
Starting Page	83
Ending Page	91
Page Count	9
File Format	PDF
ISSN	16737067
Journal	Neuroscience Bulletin
Volume Number	23
Issue Number	2
e-ISSN	19958218
Language	Chinese
Publisher	Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Publisher Date	2008-02-01
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	epilepsy development cell proliferation learning memory glucocorticoid Pain Medicine Neurology Anatomy Anesthesiology Human Physiology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Neuroscience Physiology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Changes of serum adrenocorticotropic hormone and cortisol levels during sleep seizures

Inhibiting effect of vagal nerve stimulation to seizures in epileptic process of rats

Generation of Febrile Seizures and Subsequent Epileptogenesis

Interleukin-1β with learning and memory

Diagnostic value of amplitude-integrated electroencephalogram in neonatal seizures

Early hypoactivity of hippocampal rhythms during epileptogenesis after prolonged febrile seizures in freely-moving rats

Gender difference in acquired seizure susceptibility in adult rats after early complex febrile seizures

Effects of synaptic plasticity regulated by 17β-estradiol on learning and memory in rats with Alzheimer’s disease

Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats

Morphological and behavioral consequences of recurrent seizures in neonatal rats are associated with glucocorticoid levels

Similar Documents

Changes of serum adrenocorticotropic hormone and cortisol levels during sleep seizures

Inhibiting effect of vagal nerve stimulation to seizures in epileptic process of rats

Generation of Febrile Seizures and Subsequent Epileptogenesis

Interleukin-1β with learning and memory

Diagnostic value of amplitude-integrated electroencephalogram in neonatal seizures

Early hypoactivity of hippocampal rhythms during epileptogenesis after prolonged febrile seizures in freely-moving rats

Gender difference in acquired seizure susceptibility in adult rats after early complex febrile seizures

Effects of synaptic plasticity regulated by 17β-estradiol on learning and memory in rats with Alzheimer’s disease

Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats

Morphological and behavioral consequences of recurrent seizures in neonatal rats are associated with glucocorticoid levels