NDLI: Biocalorimetric and respirometric studies on metabolic activity of aerobically grown batch culture ofPseudomonas aeruginosa

Content Provider	Springer Nature Link
Author	Sivaprakasam, Senthilkumar Mahadevan, Surianarayanan Bhattacharya, Madhuchhanda
Copyright Year	2007
Abstract	Biocalorimetry has proved to be a useful tool for scale up and control of bioreactors. The findings reported here are fundamental data required for scale up and control of a reactor for the treatment of saline tannery wastewater. The study deals with biokinetics of a halo-tolerant bacteriaPseudomonas aeruginosa isolated from tannery saline wastewater (soak liquor). Batch experiments were performed in a biocalorimeter and the isolated strain was grown in a glucose-limited mineral salt medium (MSM) at optimized growth conditions. Tessier model is found to fit well for the growth ofP. aeruginosa in biocalorimeter. Biokinetic constants are evaluated and simulation is done to validate experimental results with theoretical values. Respirogram and heat profiles are seen to follow the biomass growth curve. Oxycalorific coefficient is validated with the theoretical values and those noticed in the published literature. There is a good correlation between experimentally determined heat yields and the theoretical values predicted by elemental and enthalpy balances. The heat yield and biomass yield values indicated the behavior of the isolated organism in a substrate-limited well defined growth media (MSM)
Starting Page	340
Ending Page	347
Page Count	8
File Format	PDF
ISSN	12268372
Journal	Biotechnology and Bioprocess Engineering
Volume Number	12
Issue Number	4
Language	English
Publisher	The Korean Society for Biotechnology and Bioengineering
Publisher Date	2007-01-01
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	biocalorimetry Pseudomonas aeruginosa metabolic activity Tessier model oxygen uptake Biotechnology Industrial and Production Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology

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