NDLI: Comparative analysis of explosive RDX-induced proteomes in the Pseudomonas sp. HK-6 wild-type strain and its rpoH mutant strain

Content Provider	Springer Nature Link
Author	Lee, Bheong Uk Choi, Moon Seop Oh, Kye Heon
Copyright Year	2013
Abstract	Pseudomonas sp. HK-6 can utilize the explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) as the sole nitrogen source under aerobic conditions. It is known that HK-6 is capable of completely degrading 50 μM RDX within 50 days, while the rpoH mutant degrades less than 10% of that amount in the same period of time. The proteomes of the HK-6 and the rpoH mutant strains grown under RDX stress conditions were compared using 2-dimensional electrophoresis (2-DE). A total of 14 upregulated and down-regulated unambiguous protein spots were analyzed using MALDI-TOF MS. Several down-regulated proteins connected with energy metabolism, including NirB, RimO, and NahH, and a transport and binding protein (AapJ) were less expressed in the rpoH genetic background than in the wild-type, and certain proteins connected with the cell envelope, including OprQ and Alg8, were more highly expressed in the rpoH mutant than in the wild-type. It was shown that certain proteins such as GroEL were not expressed in rpoH cells. These results provide insight into survival and the role of the rpoH gene for RDX degradation under RDX stress conditions. In addition to the proteome analysis, the 16S rRNA of HK-6 was cloned and sequenced to draw a phylogenetic tree for precise species identification. The 16S rRNA sequence of HK-6 is closely related to that of Pseudomonas putida.
Starting Page	1224
Ending Page	1229
Page Count	6
File Format	PDF
ISSN	12268372
Journal	Biotechnology and Bioprocess Engineering
Volume Number	18
Issue Number	6
e-ISSN	19763816
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-01-04
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	RDX proteomes rpoH mutant Pseudomonas sp. HK-6 Biotechnology Industrial and Production Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comparative analysis of explosive RDX-induced proteomes in the Pseudomonas sp. HK-6 wild-type strain and its rpoH mutant strain

Comparative analysis of 2,4,6-trinitrotoluene (TNT)-induced cellular responses and proteomes in Pseudomonas sp. HK-6 in two types of media

Integrative analysis of cellular responses of Pseudomonas sp. HK-6 to explosive RDX using its xenA knockout mutant

Characterization of Pseudomonas sp. HK-6 cells responding to explosive RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine)

Purification and characterization of the NAD(P)H-nitroreductase for the catabolism of 2,4,6-trinitrotoluene (TNT) inPseudomonas sp. HK-6

Characterization and Proteomic Analysis of the Pseudomonas sp. HK-6 xenB Knockout Mutant Under RDX (Hexahydro-1,3,5-trinitro-1,3,5-triazine) Stress

Strain improvement and metabolic flux modeling of wild-type and mutant Alcaligenes sp. NX-3 for synthesis of exopolysaccharide welan gum

Biological removal of explosive 2,4,6-trinitrotoluene byStenotrophomonas sp. OK-5 in bench-scale bioreactors

Use of an algD Promoter-Driven Expression System for the Degradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Pseudomonas sp. HK-6

Comparative analysis of explosive RDX-induced proteomes in the Pseudomonas sp. HK-6 wild-type strain and its rpoH mutant strain

Similar Documents

Comparative analysis of explosive RDX-induced proteomes in the Pseudomonas sp. HK-6 wild-type strain and its rpoH mutant strain

Comparative analysis of 2,4,6-trinitrotoluene (TNT)-induced cellular responses and proteomes in Pseudomonas sp. HK-6 in two types of media

Integrative analysis of cellular responses of Pseudomonas sp. HK-6 to explosive RDX using its xenA knockout mutant

Characterization of Pseudomonas sp. HK-6 cells responding to explosive RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine)

Purification and characterization of the NAD(P)H-nitroreductase for the catabolism of 2,4,6-trinitrotoluene (TNT) inPseudomonas sp. HK-6

Characterization and Proteomic Analysis of the Pseudomonas sp. HK-6 xenB Knockout Mutant Under RDX (Hexahydro-1,3,5-trinitro-1,3,5-triazine) Stress

Strain improvement and metabolic flux modeling of wild-type and mutant Alcaligenes sp. NX-3 for synthesis of exopolysaccharide welan gum

Biological removal of explosive 2,4,6-trinitrotoluene byStenotrophomonas sp. OK-5 in bench-scale bioreactors

Use of an algD Promoter-Driven Expression System for the Degradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by Pseudomonas sp. HK-6

Comparative analysis of explosive RDX-induced proteomes in the Pseudomonas sp. HK-6 wild-type strain and its rpoH mutant strain