NDLI: Insulin Prevents Leptin Inhibition of RM1 Prostate Cancer Cell Growth

Content Provider	Springer Nature Link
Author	Ribeiro, Andreia M. Pereira, Sofia Andrade, Sara Costa, Madalena Lopes, Carlos Aguas, Artur P. Monteiro, Mariana P.
Copyright Year	2011
Abstract	The association between obesity and cancer is controversial: whereas several epidemiological, clinical and research studies using cancer cell lines have supported that high levels of insulin and leptin could favor prostate cancer development and dissemination, other studies have demonstrated opposite effects or even absence of association. The main goal of this study was to evaluate the in vitro proliferation of murine androgen insensitive prostate carcinoma cells RM1 in the presence of leptin and insulin. After assessing and confirming the presence of leptin and insulin receptors in RM1 cells by immunocytochemistry, cells were cultured in the presence of different concentrations of leptin (0, 25, 50, 100 and 200 ng/mL), insulin (0, 50, 100, 150 and 200 nM) or leptin plus insulin ( 25 ng/ml + 50 nM; 50 ng/ml + 100 nM; 100 ng/ml + 150 nM; 200 ng/ml + 200 nM; 25 ng/ml + 150 nM; 100 ng/ml + 50 nM of leptin plus insulin, respectively). Cell proliferation was evaluated by assessing the percentage of resazurin reduction, a surrogate marker of cell metabolic rate. Leptin significantly decreased the percentage of resazurin reduction in all studied concentrations while there was only a slight or non significant difference in RM1cell proliferation in the presence of insulin or insulin combined with leptin when compared with control. These results show that leptin decreases RM1 prostate cancer cell proliferation at the studied concentrations, while insulin is able to antagonize the leptin inhibition of RM1 prostate cancer cell growth in vitro. The difference in cell growth that is modulated by the various hormonal environments may explain the heterogeneous behavior of prostate cancer in the obese human population.
Starting Page	499
Ending Page	507
Page Count	9
File Format	PDF
ISSN	12194956
Journal	Pathology & Oncology Research
Volume Number	18
Issue Number	2
e-ISSN	15322807
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-11-24
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Prostate cancer Obesity Leptin Insulin RM1 cells Cellular proliferation Biomedicine general Immunology Oncology Cancer Research Pathology
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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