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Virologica Sinica : Volume 27, Issue 4, August 2012

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Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays

Content Provider	Springer Nature Link
Author	Huang, Yi Wei, Hongping Wang, Yunpeng Shi, Zhengli Raoul, Herve Yuan, Zhiming
Copyright Year	2012
Abstract	Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus (ZEBOV) and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 10$^{3}$ to 10$^{9}$, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 10$^{10}$ RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable for future surveillance and specific diagnosis of ZEBOV and MARV in China.
Starting Page	273
Ending Page	277
Page Count	5
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	27
Issue Number	5
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2012-09-21
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Ebola virus Marburg virus Nucleoprotein (NP) gene TaqMan RT-PCR Microbial Genetics and Genomics Medical Microbiology Virology Biochemistry Oncology Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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Laboratory detection and diagnosis of filoviruses

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Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays