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Evaluation of a direct rapid immunohistochemical test (dRIT) for rapid diagnosis of rabies in animals and humans

Content Provider	Springer Nature Link
Author	Madhusudana, Shampur Narayan Subha, Sundaramurthy Thankappan, Ullas Ashwin, Yajaman Belludi
Copyright Year	2012
Abstract	Presently the gold standard diagnostic technique for rabies is the direct immunofluorescence assay (dFA) which is very expensive and requires a high level of expertise. There is a need for more economical and user friendly tests, particularly for use in developing countries. We have established one such test called the direct rapid immunohistochemical test (dRIT) for diagnosis of rabies using brain tissue. The test is based on capture of rabies nucleoprotein (N) antigen in brain smears using a cocktail of biotinylated monoclonal antibodies specific for the N protein and color development by streptavidin peroxidase-amino ethyl carbazole and counter staining with haematoxollin. The test was done in parallel with standard FAT dFA using 400 brain samples from different animals and humans. The rabies virus N protein appears under light microscope as reddish brown particles against a light blue background. There was 100 % correlation between the results obtained by the two tests. Also, interpretation of results by dRIT was easier and only required a light microscope. To conclude, this newly developed dRIT technique promises to be a simple, cost effective diagnostic tool for rabies and will have applicability in field conditions prevalent in developing countries.
Starting Page	299
Ending Page	302
Page Count	4
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	27
Issue Number	5
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2012-10-11
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Rabies Post-mortem diagnosis Fluorescent antibody technique Immunohistochemistry Direct rapid immunohistochemical test (dRIT) Microbial Genetics and Genomics Medical Microbiology Virology Biochemistry Oncology Microbiology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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