NDLI: Effects of rat cytomegalovirus on the nervous system of the early rat embryo

Content Provider	Springer Nature Link
Author	Sun, Xiuning Guan, YingJun Li, Fengjie Li, Xutong Wang, Xiaowen Guan, Zhiyu Sheng, Kai Yu, Li Liu, Zhijun
Copyright Year	2012
Abstract	The purpose of the study was to investigate the impact of rat cytomegalovirus (RCMV) infection on the development of the nervous system in rat embryos, and to evaluate the involvement of Wnt signaling pathway key molecules and the downstream gene neurogenin 1 (Ngn1) in RCMV infected neural stem cells (NSCs). Infection and control groups were established, each containing 20 pregnant Wistar rats. Rats in the infection group were inoculated with RCMV by intraperitoneal injection on the first day of pregnancy. Rat E20 embryos were taken to evaluate the teratogenic rate. NSCs were isolated from E13 embryos, and maintained in vitro. We found: 1) Poor fetal development was found in the infection group with low survival and high malformation rates. 2) The proliferation and differentiation of NSCs were affected. In the infection group, NSCs proliferated more slowly and had a lower neurosphere formation rate than the control. The differentiation ratio from NSCs to neurons and glial cells was significantly different from that of the control, showed by immunofluorescence staining. 3) Ngn1 mRNA expression and the nuclear β-catenin protein level were significantly lower than the control on day 2 when NSCs differentiated. 4) The Morris water maze test was performed on 4-week pups, and the infected rats were found worse in learning and memory ability. In a summary, RCMV infection caused abnormalities in the rat embryonic nervous system, significantly inhibited NSC proliferation and differentiation, and inhibited the expression of key molecules in the Wnt/β-catenin signaling pathway so as to affect NSCs differentiation. This may be an important mechanism by which RCMV causes embryonic nervous system abnormalities.
Starting Page	234
Ending Page	240
Page Count	7
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	27
Issue Number	4
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2012-07-28
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	RCMV NSCs Proliferation and differentiation Wnt/β-catenin Ngn1 Microbiology Virology Biochemistry Medical Microbiology Oncology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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