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| Content Provider | Springer Nature Link |
|---|---|
| Author | Han, Xue Ying, Xiaoling Huang, Hao Zhou, Shili Huang, Qi |
| Copyright Year | 2012 |
| Abstract | Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease (HFMD) resulting in hundreds of deaths of children every year; However, currently, there is no effective treatment for EV71. In this study, the EV71 poly-protein (EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115. The EV71 P1 protein with a molecular weight of 100 kD was produced and secreted into the medium. The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%. The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits. We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection. |
| Starting Page | 254 |
| Ending Page | 258 |
| Page Count | 5 |
| File Format | |
| ISSN | 16740769 |
| Journal | Virologica Sinica |
| Volume Number | 27 |
| Issue Number | 4 |
| e-ISSN | 1995820X |
| Language | English |
| Publisher | SP Wuhan Institute of Virology, CAS |
| Publisher Date | 2012-07-28 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | EV71-P1 protein Expression Purification Immunogenicity Pichia pastoris Microbiology Virology Biochemistry Medical Microbiology Oncology Microbial Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology Immunology Molecular Medicine |
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