NDLI: Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70

Content Provider	Springer Nature Link
Author	Tian, Hong Wu, Jing yan Shang, You jun Ying, Shuang hui Zheng, Hai xue Liu, Xiang tao
Copyright Year	2010
Abstract	VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore, anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.
Starting Page	206
Ending Page	212
Page Count	7
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	25
Issue Number	3
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2010-06-06
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Swine vesicular disease virus Capsid protein precursor gene (vp1) Gene expression Immune response Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70