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| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, Jian qiang Cheng, Jie Lan, Xi Li, Xue rui Li, Wei Yin, Xiang ping Li, Bao yu Yang, Bin Li, Zhi yong Zhang, Yun Liu, Ji xing |
| Copyright Year | 2010 |
| Abstract | The complete genome sequence of transmissible Gastroenteritis virus (TGEV) strain TS, previously isolated from Gansu province, was cloned and compared with published sequence data from other TGEV strains. Phylogenetic tree analysis based on the amino acid and nucleotide sequences of the S gene showed that the TGEV strains were divided into 3 clusters. TGEV TS showed a close evolutionary relationship to the American Miller cluster but had a 5′ non-translated region (NTR) sequence closely related to the American Purdue cluster. Continued culture in different cell types indicated that TGEV TS virulence could be attenuated after fifty passages in Porcine kidney (PK-15) cells, and that the Porcine kidney cell line IB-RS-2 (IBRS) was not suitable for culture of the TGEV strain TS. |
| Starting Page | 213 |
| Ending Page | 224 |
| Page Count | 12 |
| File Format | |
| ISSN | 16740769 |
| Journal | Virologica Sinica |
| Volume Number | 25 |
| Issue Number | 3 |
| e-ISSN | 1995820X |
| Language | English |
| Publisher | SP Wuhan Institute of Virology, CAS |
| Publisher Date | 2010-06-06 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Cloning Complete genome Gastroenteritis virus (TGEV) Cell culture Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology Immunology Molecular Medicine |
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