NDLI: Quantification of Simian immunodeficiency virus by SYBR green RT-PCR technique

Content Provider	Springer Nature Link
Author	Lu, Jing Qin, Li Liu, Guang jie Zhao, Si ting Chen, Xiao ping
Copyright Year	2008
Abstract	Plasma viral RNA load is widely accepted as the most relevant parameter to assess the status and progression of Simian immunodeficiency virus (SIV) infections. To accurately measure RNA levels of the virus, a one-step fluorescent quantitative assay was established based on the SYBR green Real-time reverse transcription-polymerase chain reaction (RT-PCR). The lower detection limit of the assay was 10 copies per reaction for the virus. This method was successfully applied to quantify SIVmac251 and SIVmac239 viruses produced in CEM×174 cells. Additionally, the performance of the SYBR green RT-PCR was assessed in a SIVmac251 infected rhesus macaque. The result demonstrated that the method could detect as little as 215 copies per milliliter of plasma and the dynamic pattern of viral load was highly consistent with previous results. With regard to convenience, sensitivity and accuracy our assay represents a realistic alternative to both branched-chain DNA (b-DNA) assays or real-time PCR assays based on TaqMan probes.
Starting Page	189
Ending Page	195
Page Count	7
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	23
Issue Number	3
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2008-07-18
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Real time PCR (RT-PCR) SYBR green Simian immunodeficiency virus (SIV) Viral loads Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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