NDLI: A novel β-glucosidase with lipolytic activity from a soil metagenome

Content Provider	Springer Nature Link
Author	Jiang, Cheng Jian Chen, Gao Huang, Jie Huang, Qin Jin, Ke Shen, Pei Hong Li, Jun Fang Wu, Bo
Copyright Year	2011
Abstract	Moonlighting proteins have two different functions within a single polypeptide chain. Exploring moonlighting enzymes from the environment using the metagenomic approach is interesting. In the present study, a novel β-glucosidase gene, designated as bgl1D, with lipolytic activity (renamed Lip1C) was cloned through function-based screening of a metagenomic library from uncultured soil microorganisms. The deduced amino acid sequence comparison and phylogenetic analysis also indicated that Lip1C and other putative lipases are closely related. Biochemical characterization demonstrated that the maximum activity of the recombinant Lip1C protein occurs at pH 8.0 and 30°C using 4-nitrophenyl butyrate as substrate. The putative lipase had an apparent K $_{m}$ value of 0.88 mmol/L, a k $_{cat}$ value of 212/min, and a k $_{cat}$/K $_{m}$ value of 241 L/mmol/min. Lip1C exhibited habitat-specific characteristics with 5 mmol/L AlCl$_{3}$, CuCl$_{2}$, and LiCl. The characterization of the biochemical properties of Lip1C enhances our understanding of this novel moonlighting enzyme isolated from a soil metagenome.
Starting Page	563
Ending Page	570
Page Count	8
File Format	PDF
ISSN	00155632
Journal	Folia Microbiologica
Volume Number	56
Issue Number	6
e-ISSN	18749356
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-11-25
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Environmental Engineering/Biotechnology Immunology Applied Microbiology Microbiology Life Sciences
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology

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