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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Chun Pang, Victor Fei Jeng, Chian Ren Lee, Fan Huang, Yu Wen Lin, Yeou Liang Hsiao, Shih Hsuan Lai, Shiow Suey |
| Copyright Year | 2011 |
| Abstract | A loop-mediated isothermal amplification (LAMP) method with a real-time monitoring system was developed for the detection of porcine circovirus type 1 (PCV1) in commercial swine vaccines. This method was highly specific for PCV1. No cross-reaction to porcine circovirus type 2, porcine parvovirus, pseudorabies virus, classical swine fever virus, and porcine reproductive and respiratory syndrome virus was observed. The analytical sensitivity of the LAMP for PCV1 DNA was 10 copies/μl in the case of positive recombinant plasmid comparable to that obtained from the nested polymerase chain reaction (nested PCR). Furthermore, 25 commercial swine vaccines were tested by both the LAMP and the nested PCR, and three of them were tested positive for PCV1 DNA. These results indicate that PCV1 DNA can be real-time detected by the LAMP; the method was highly specific, sensitive, and rapid for the detection of PCV1 DNA, particularly in commercial swine vaccines. |
| Starting Page | 483 |
| Ending Page | 489 |
| Page Count | 7 |
| File Format | |
| ISSN | 00155632 |
| Journal | Folia Microbiologica |
| Volume Number | 56 |
| Issue Number | 6 |
| e-ISSN | 18749356 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2011-09-27 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Environmental Engineering/Biotechnology Immunology Applied Microbiology Microbiology Life Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology |
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