NDLI: Anthocyanin Profiling Method Based on Isocratic Elution for Comparable Speed, Reproducibility, and Quantitation with Gradient Elution

Content Provider	Springer Nature Link
Author	Singh, Jashbir Sharma, Khushbu Walia, Suresh Saha, Supradip
Copyright Year	2016
Abstract	The present work aims at developing a simple and rapid method for the quantification of anthocyanins irrespective of acylation present or not. The proposed analytical approach is based on isocratic elution mode in order to avoid the complexity of gradient elution system. The optimal conditions for the separation of anthocyanins were obtained as 14 % acetonitrile in water containing 2 % trifluroacetic acid with a flow rate of 0.6 ml min$^{−1}$ when analyzed in Syzygium cumini (Jamun) anthocyanins. The method was successfully separated eight anthocyanins present in Jamun using C$_{18}$ column as a stationary phase. Anthocyanins characterization was done by using electrospray ionization mass spectrometry (ESI-MS). LOD and LOQ value was calculated based on signal-to-noise ratio (S/N ratio) and ordinary least square regression (OLSR) approaches. LOD were obtained as 0.9, 0.4, and 0.3 μg ml$^{−1}$ by S/N ratio method and 0.27, 1.2, and 1.0 μg ml$^{−1}$unit by OLSR method for M$_{3}$-G, C$_{3}$-G and D$_{3}$-G, respectively. In the isocratic elution mode, critical pair resolution was 1.95 whereas it was 0.85 under the gradient elution mode. Interestingly, ratio of peak area and peak height in gradient and isocratic elution was virtually same for almost all the evaluated peaks. More importantly, the method was validated with other three sources of anthocyanins (black carrot, red cabbage, and berry) and found variable resolution and response. After analyzing all the data, it may be concluded that the proposed method is useful for separation and identification of anthocyanin irrespective of acylation present in it when the number of anthocyanin is ≤10.
Starting Page	118
Ending Page	128
Page Count	11
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	10
Issue Number	1
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2016-06-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anthocyanins Liquid chromatographic method Isocratic elution Acylation Gradient elution Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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