NDLI: Quantitative Determination of Butocarboxim in Agricultural Products Based on Biotinylated Monoclonal Antibody

Content Provider	Springer Nature Link
Author	Fang, Qingkui Wang, Limin Cheng, Qi Wang, Yulong Wang, Suyan Cai, Jia Liu, Fengquan
Copyright Year	2014
Abstract	The carbamate pesticides, such as butocarboxim, are considered hazardous substances because of their toxicity to non-target species and their persistence in the environment and agricultural products. Thus, it is highly desirable to design a sensitive and rapid detection of butocarboxim for the safety of agriculture products. In this study, a new hapten of butocarboxim was designed and synthesized; a highly affinitive and selective monoclonal antibody (mAb) against butocarboxim was produced, which was obtained from immunized BALB/c female mice. Based on the biotinylated mAb, an indirect competitive enzyme-linked immunosorbent assay (bi-ELISA) and an indirect competitive electrochemical immunoassay (bi-ECIA) for detecting butocarboxim pesticides in agricultural products were developed. Under optimized experiment conditions, biotin with an amount of 20-fold molar excess against mAb was used, and meanwhile, for bi-ECIA, the concentration of coating antigen is two times lower than that of bi-ELISA (0.031 and 0.062 μg mL$^{−1}$, respectively), the concentration of mAb is four times lower than that of bi-ELISA (0.07 and 0.28 μg · mL$^{−1}$, respectively). Depending on the assay format, the limits of detection (LOD) of bi-ECIA and bi-ELISA were 1.41 and 7.55 ng mL$^{−1}$, respectively. The results showed that the sensitivity (LOD) of bi-ECIA was over five times higher than that of bi-ELISA. At the same time, the recoveries obtained by standard butocarboxim addition to apple and greengrocery samples were from 89.6 to 108.5 % for bi-ELISA and from 87.2 to 109.8 % for bi-ECIA.
Starting Page	1248
Ending Page	1257
Page Count	10
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	8
Issue Number	5
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2014-10-02
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Butocarboxim A new hapten Biotinylated mAb bi-ELISA bi-ECIA Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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