NDLI: Comparative Electrochemical Determination of Total Antioxidant Activity in Infant Formula with Breast Milk

Content Provider	Springer Nature Link
Author	Lugonja, Nikoleta M. Stanković, Dalibor M. Spasić, Snezana D. Roglić, Goran M. Majlović, Dragan D. Vrvić, Miroslav M.
Copyright Year	2013
Abstract	The aim of this study is to investigate the significance of breast milk and infant formula in the prevention of oxidative stress, by electrochemical determination of the total antioxidant potential, demonstrating the relationship between the antioxidant capacity of milk and postnatal age. Human breast milk, commercial UHT milk, and infant formulas supplemented with prebiotics were used. Samples were diluted in phosphate buffer solution, and the total antioxidant activity was potentiometrically measured by the iodine/iodide redox couple with the Pt Fisher electrode as a working electrode and saturated calomel as a reference electrode. Cyclic voltammograms and differential pulse voltammograms were recorded with the glassy carbon electrode as the working electrode, an accessory platinum electrode, and an Ag/AgCl reference electrode. The potentiometric measurement indicates that human breast milk has the highest redox potential, while the commercial UHT milk has very low potential. Infant formulas also have high potential. The main advantage of electrochemical methods used to assess the total antioxidant activity of milk was that they directly monitored the electron-donating ability of the compounds and could be used for the quantitative analysis of the total antioxidants of different types of milk.
Starting Page	337
Ending Page	344
Page Count	8
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	7
Issue Number	2
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2013-05-04
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Total antioxidant activity Infant formula Human breast milk Potentiometry Cyclic voltammetry Differential pulse voltammetry Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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