NDLI: Antioxidant Capacity of Hydrophilic Food Matrices: Optimization and Validation of ORAC Assay

Content Provider	Springer Nature Link
Author	Kevers, Claire Sipel, Arnaud Pincemail, Joël Dommes, Jacques
Copyright Year	2013
Abstract	It is widely accepted that ORAC is a useful method for assessing food extracts that contain various antioxidants. The principal aim of this study was to validate the ORAC assay. We first identified parameters that can interfere with the ORAC assay and we optimized it. Then, experiments were conducted to determine the limits of linearity and response function, to determine the accuracy profiles to circumvent some of the drawbacks of traditional validation procedures. Trueness, selectivity and limits of quantification of the method were also determined. Our objective of ORAC method validation is thus to give guarantees that most of the results generated during use of this method will be close enough to unknown true value of antioxidant capacity of food matrices. The validation results indicate that the described method will give accurate and reliable results for Trolox equivalent values ranging from 50 to 200 μmol/L.
Starting Page	409
Ending Page	416
Page Count	8
File Format	PDF
ISSN	19369751
Journal	Food Analytical Methods
Volume Number	7
Issue Number	2
e-ISSN	1936976X
Language	English
Publisher	Springer US
Publisher Date	2013-05-25
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antioxidant capacity Optimization ORAC Validation Accuracy profiles Food matrices Food Science Chemistry/Food Science Microbiology Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Safety Research Safety, Risk, Reliability and Quality Applied Microbiology and Biotechnology Food Science

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