NDLI: Improving Saccharification Efficiency of Alfalfa Stems Through Modification of the Terminal Stages of Monolignol Biosynthesis

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Improving Saccharification Efficiency of Alfalfa Stems Through Modification of the Terminal Stages of Monolignol Biosynthesis

Content Provider	Springer Nature Link
Author	Jackson, Lisa A. Shadle, Gail L. Zhou, Rui Nakashima, Jin Chen, Fang Dixon, Richard A.
Copyright Year	2008
Abstract	A series of transgenic lines of alfalfa (Medicago sativa) were generated in which either one of the two potentially terminal enzymes of the monolignol pathway, cinnamoyl CoA reductase (CCR) or cinnamyl alcohol dehydrogenase (CAD) was down-regulated by expression of antisense transgenes. Levels of CCR enzymatic activity were reduced to between 10% to 65% of the control level, and levels of CAD activity were similarly reduced to between 5% to 40% of the control. Biomass yields were reduced in the most strongly down-regulated lines for both transgenes, but many of the lines exhibited reduced lignin levels but normal biomass and flowering time. In vitro dry matter digestibility was increased for most transgenic lines compared to controls. Saccharification efficiency was determined by measuring the release of sugars from cell walls directly, or after sulfuric acid pre-treatment and subsequent digestion with a mixture of cellulase and cellobiase. Several CCR down-regulated lines had significantly enhanced saccharification efficiency with both pre-treated and untreated tissues, whereas CAD down-regulation had less impact on sugar release when compared to that from CCR lines with similar lignin contents. One CCR line with a 50–60% improvement in saccharification efficiency exhibited normal biomass production, indicating the potential for producing high yielding, improved feedstocks for bioethanol production through genetic modification of the monolignol pathway.
Starting Page	180
Ending Page	192
Page Count	13
File Format	PDF
ISSN	19391234
Journal	BioEnergy Research
Volume Number	1
Issue Number	3-4
e-ISSN	19391242
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-09-27
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Alfalfa Genetic modification Lignin pathway Saccharification Transgenic plants Wood Science & Technology Plant Ecology Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Renewable Energy, Sustainability and the Environment Agronomy and Crop Science

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Improving Saccharification Efficiency of Alfalfa Stems Through Modification of the Terminal Stages of Monolignol Biosynthesis

Supplementary materials: Improving saccharification efficiency of alfalfa stems through modification of the terminal stages of monolignol biosynthesis

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Improving Saccharification Efficiency of Alfalfa Stems Through Modification of the Terminal Stages of Monolignol Biosynthesis

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