NDLI: Chemical synthesis and biological activities of 3-alkyl pyridinium polymeric analogues of marine toxins

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Chemical synthesis and biological activities of 3-alkyl pyridinium polymeric analogues of marine toxins

Content Provider	Springer Nature Link
Author	Houssen, Wael E. Lu, Zhibao Edrada Ebel, RuAngelie Chatzi, Christina Tucker, Steven J. Sepčić, Kristina Turk, Tom Zovko, Ana Shen, Sanbing Mancini, Ines Scott, Roderick H. Jaspars, Marcel
Copyright Year	2010
Abstract	Two new large poly-1,3-dodecylpyridinium salts, APS12 and APS12-2 of 12.5- and 14.7-kDa size, respectively, were synthesised and tested for their pore-forming and transfection capabilities in HEK 293 and undifferentiated mouse ES cells using patch-clamp recording, Ca$^{2+}$ imaging and flow cytometry. Polymerisation reactions were enhanced by microwaves, and the product sizes were controlled by altering the irradiation time. This method can also be applied to obtain polymers with variable linking chains as shown by the preparation of poly-(1,3-octylpyridinium) salt of 11.9-kDa size. Molecular weights of the final products were determined using ESIMS analysis, which also indicated the products to be amongst the largest macro-cycles ever recorded, up to a 900-membered ring. Anti-bacterial, haemolytic and anti-acetylcholinesterase activities were also reported for the two dodecyl pyridinium polymers. These biological activities are characteristic to the structurally related marine toxin, poly-APS.
Starting Page	113
Ending Page	125
Page Count	13
File Format	PDF
ISSN	18646158
Journal	Journal of Chemical Biology
Volume Number	3
Issue Number	3
e-ISSN	18646166
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-02-17
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Marine toxins Transfection Poly-APS Pore formers Microwave-assisted polymerisation Biochemistry Pharmacology/Toxicology Cell Biology Biophysics and Biological Physics Physical Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Biophysics

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