NDLI: Towards native-state imaging in biological context in the electron microscope

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Towards native-state imaging in biological context in the electron microscope

Content Provider	Springer Nature Link
Author	Weston, Anne E. Armer, Hannah E. J. Collinson, Lucy M.
Copyright Year	2009
Abstract	Modern cell biology is reliant on light and fluorescence microscopy for analysis of cells, tissues and protein localisation. However, these powerful techniques are ultimately limited in resolution by the wavelength of light. Electron microscopes offer much greater resolution due to the shorter effective wavelength of electrons, allowing direct imaging of sub-cellular architecture. The harsh environment of the electron microscope chamber and the properties of the electron beam have led to complex chemical and mechanical preparation techniques, which distance biological samples from their native state and complicate data interpretation. Here we describe recent advances in sample preparation and instrumentation, which push the boundaries of high-resolution imaging. Cryopreparation, cryoelectron microscopy and environmental scanning electron microscopy strive to image samples in near native state. Advances in correlative microscopy and markers enable high-resolution localisation of proteins. Innovation in microscope design has pushed the boundaries of resolution to atomic scale, whilst automatic acquisition of high-resolution electron microscopy data through large volumes is finally able to place ultrastructure in biological context.
Starting Page	101
Ending Page	112
Page Count	12
File Format	PDF
ISSN	18646158
Journal	Journal of Chemical Biology
Volume Number	3
Issue Number	3
e-ISSN	18646166
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-11-15
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Transmission electron microscopy Scanning electron microscopy Artifacts Native state Cryopreparation Cryo-EM ESEM Correlative Volume EM FIB/SEM SBF/SEM Biochemistry Pharmacology/Toxicology Cell Biology Biophysics and Biological Physics Physical Chemistry
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Biophysics

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