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| Content Provider | Springer Nature Link |
|---|---|
| Author | Thavachelvam, Kulanthaivel Tuteja, Urmil Sarika, Kumari Nagendra, Suryanarayana Kumar, Subodh |
| Copyright Year | 2012 |
| Abstract | The need for a rapid detection and characterization of biowarfare (BW) agents cannot be over emphasized. With diverse array of potential BW pathogen available presently, rapid identification of the pathogen is crucial, so that specific therapy and control measures can be initiated. We have developed a multiplex polymerase chain reaction based reverse line blot macroarray to simultaneously detect four pathogens of BW importance viz. Bacillus anthracis, Yersinia pestis, Brucella melitensis and Burkholderia pseudomallei. The multiplex PCR utilizes 14 pairs of primers targeting 18 specific markers. These markers include genes which are genus specific, species-specific chromosomal sequences and virulence markers of plasmid origin. The assay was evaluated on various human, environment and animal isolates. The assay w successful in simultaneous detection and characterization of isolates of the four pathogens on as a single platform with sensitivity ranging from 0.3 pg to 0.3 ng of genomic DNA. The assay was able to detect 5 × 10$^{2}$ cfu/ml for B. anthracis, 8 × 10$^{2}$ cfu/ml for Yersinia sp., 1.4 × 10$^{2}$ cfu/ml for B. melitensis and 4 × 10$^{2}$ cfu/ml for B. pseudomallei. |
| Starting Page | 41 |
| Ending Page | 47 |
| Page Count | 7 |
| File Format | |
| ISSN | 00468991 |
| Journal | Indian Journal of Microbiology |
| Volume Number | 53 |
| Issue Number | 1 |
| e-ISSN | 09737715 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-11-06 |
| Publisher Institution | Association of Microbiologists of India |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Reverse line blot Bacillus anthracis Yersinia pestis Brucella Burkholderia Biowarfare Microbiology Medical Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
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