NDLI: A sensitive & specific multiplex PCR assay for simultaneous detection of Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei & Brucella species

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A sensitive & specific multiplex PCR assay for simultaneous detection of Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei & Brucella species

Content Provider	Semantic Scholar
Author	Batra, Sai Arun Sreerama Krupanidhi Tuteja, Urmil
Copyright Year	2013
Abstract	BACKGROUND & OBJECTIVES Bacillus anthracis, Yersinia pestis, Burkholderia pseudomallei and Brucella species are potential biowarfare agents. Classical bacteriological methods for their identification are cumbersome, time consuming and of potential risk to the handler. METHODS We describe a sensitive and specific multiplex polymerase chain reaction (mPCR) assay involving novel primers sets for the simultaneous detection of B. anthracis, Y. pestis, B. pseudomallei and Brucella species. An additional non-competitive internal amplification control (IAC) was also included. RESULTS The mPCR was found to be specific when tested against closely related organisms. The sensitivity of the assay in spiked blood samples was 50 colony forming units (cfus)/25 μl reaction, for the detection of B. anthracis, Y. pestis and Brucella species; and 150 cfus/25 μl reaction, for B. pseudomallei. The assay proved useful in correctly and promptly identifing the clinical isolates of the targeted agents recovered from patients, compared to the gold standard culture methods. INTERPRETATION & CONCLUSION The assay described in this study showed promise to be useful in application as a routine detection cum diagnostic method for these pathogens.
Starting Page	111
Ending Page	116
Page Count	6
File Format	PDF HTM / HTML
PubMed reference number	24056564
Journal	Medline
Volume Number	138
Alternate Webpage(s)	http://icmr.nic.in/ijmr/2013/July/0715.pdf
Journal	The Indian journal of medical research
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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