NDLI: Differential expression of thermophilic phosphatases in the wild type and auxotrophic mutant strains of Thermoactinomyces vulgaris

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Differential expression of thermophilic phosphatases in the wild type and auxotrophic mutant strains of Thermoactinomyces vulgaris

Content Provider	Springer Nature Link
Author	Singh, V. P. Kedia, V. K. Mohanta, H. S.
Copyright Year	2007
Abstract	In the wild type strain (stock no. 1227) of Thermoactinomyces vulgaris, as reported earlier [Sinha and Singh (1980) Biochem. J. 190, 457–460], all phosphatase isoenzymes (three alkaline — AlpI, AlpII and AlpIII, and one acidic — Acp) are present. However, the auxotrophic mutants, the strains 1286 (thi $^{−}$), 1279 (nic $^{−}$, ura $^{−}$) and 1278 (thi $^{−}$, ura $^{−}$) exhibited two alkaline phosphatase isoenzymes (AlpII and AlpIII), but AlpI was lacking. In the strain 1261 (nic $^{−}$, thi $^{−}$), only AlpIII was expressed, and AlpI and AlpII isoenzymes were missing. The results suggest that the strains, which require either thiamine (1286 and 1278) or nicotinamide (1279) for their growth, were AlpI $^{−}$ mutants; and the strain (1261), which requires both thiamine and nicotinamide for its growth, was AlpI $^{−}$/AlpII $^{−}$ double mutant. There was no direct correlation between uracil auxotrophy and the expression of phosphatases. The uniform expression of AlpIII and Acp in all the strains, irrespective of their nutrient requirements, suggest that these constitutive phosphatases are species-specific. The specific activities of the thermophilic acid and alkaline phosphatases were maximum in the wild type strain (1227) of T. vulgaris. The next in phosphatase activity was the strain 1279 (an AlpI $^{−}$ mutant), followed by their decrease, in order, in the strains 1286 and 1278 (which were also AlpI $^{−}$ mutants); while least activity of these enzymes was observed in the obligate thermophile strain 1261 (AlpI $^{−}$/AlpII $^{−}$ double mutant).
Starting Page	81
Ending Page	85
Page Count	5
File Format	PDF
ISSN	00468991
Journal	Indian Journal of Microbiology
Volume Number	47
Issue Number	1
e-ISSN	09737715
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-06-14
Publisher Institution	Association of Microbiologists of India
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	auxotrophic mutants obligate thermophile phosphatase isoenzymes phosphatase mutations Thermoactinomyces vulgaris Medical Microbiology Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology

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