NDLI: The Role of Neuroglobin in the Neuroprotection of Limb Ischemic Preconditioning in Rats

Content Provider	Springer Nature Link
Author	Li, Shu Qin Li, Wen Bin Zhang, Min Wu, Yu Zhou Hu, Yu Yan
Copyright Year	2012
Abstract	Recent evidence suggests that limb ischemic preconditioning (LIP) protects neurons against cerebral ischemia-reperfusion injury. However, the mechanisms of LIP are not well understood. Neuroglobin (Ngb) is a recently discovered globin that affords protection against hypoxic/ischemic brain injury. This study was performed to investigate the role of Ngb in the neuroprotection of LIP against brain ischemia and the involvements of mitochondria in the process. The rat global brain ischemic model was used, and the CA1 hippocampus was selected as the observational target. Ngb expression was investigated by RT-PCR and Western blot. Neuropathological evaluation was performed by thionin staining. Mitochondrial membrane potential (Δψm), Na$^{+}$-K$^{+}$-ATPase activity, and ultrastructure were examined by flow cytometry, spectrophotometry, and transmission electron microscopy, respectively. We also used Ngb antisense oligodeoxynucleotides (AS-ODNs) and Ngb inducer hemin to inhibit or mimic the effect of LIP. We found that LIP significantly up-regulated Ngb expression and protected neurons against ischemia. Furthermore, LIP effectively improved deterioration in the Δψm, mitochondrial Na$^{+}$-K$^{+}$-ATPase activity, and ultrastructure induced by cerebral ischemia. These effects of LIP were inhibited partly by Ngb AS-ODNs and mimicked by hemin. It could be concluded that up-regulation of Ngb expression played an important role in the neuroprotection induced by LIP, and the Ngb-mediated neuroprotection of LIP was, at least partly, associated with mitochondria.
Starting Page	197
Ending Page	208
Page Count	12
File Format	PDF
ISSN	08937648
Journal	Molecular Neurobiology
Volume Number	47
Issue Number	1
e-ISSN	15591182
Language	English
Publisher	Humana Press Inc
Publisher Date	2012-11-21
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Limb ischemic preconditioning Brain ischemia Neuroglobin Hemin Mitochondria Neurosciences Neurobiology Cell Biology Neurology
Content Type	Text
Resource Type	Article
Subject	Neurology Cellular and Molecular Neuroscience

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The Role of Nitric Oxide in the Neuroprotection of Limb Ischemic Preconditioning in Rats

The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury

Cerebral Ischemic Preconditioning: the Road So Far…

Splenic responses play an important role in remote ischemic preconditioning-mediated neuroprotection against stroke

Cellular and Molecular Neurobiology of Brain Preconditioning

Ischemic Preconditioning Provides Neuroprotection by Induction of AMP-Activated Protein Kinase-Dependent Autophagy in a Rat Model of Ischemic Stroke

14-3-3γ and Neuroglobin are New Intrinsic Protective Factors for Cerebral Ischemia

Chronic Metformin Preconditioning Provides Neuroprotection via Suppression of NF-κB-Mediated Inflammatory Pathway in Rats with Permanent Cerebral Ischemia

Osteopontin Mediates Hyperbaric Oxygen Preconditioning-Induced Neuroprotection Against Ischemic Stroke

The Role of Neuroglobin in the Neuroprotection of Limb Ischemic Preconditioning in Rats

Similar Documents

The Role of Nitric Oxide in the Neuroprotection of Limb Ischemic Preconditioning in Rats

The Role of SUMO-Conjugating Enzyme Ubc9 in the Neuroprotection of Isoflurane Preconditioning Against Ischemic Neuronal Injury

Cerebral Ischemic Preconditioning: the Road So Far…

Splenic responses play an important role in remote ischemic preconditioning-mediated neuroprotection against stroke

Cellular and Molecular Neurobiology of Brain Preconditioning

Ischemic Preconditioning Provides Neuroprotection by Induction of AMP-Activated Protein Kinase-Dependent Autophagy in a Rat Model of Ischemic Stroke

14-3-3γ and Neuroglobin are New Intrinsic Protective Factors for Cerebral Ischemia

Chronic Metformin Preconditioning Provides Neuroprotection via Suppression of NF-κB-Mediated Inflammatory Pathway in Rats with Permanent Cerebral Ischemia

Osteopontin Mediates Hyperbaric Oxygen Preconditioning-Induced Neuroprotection Against Ischemic Stroke

The Role of Neuroglobin in the Neuroprotection of Limb Ischemic Preconditioning in Rats