NDLI: Isolation of RNA from Field-Grown Jute (Corchorus capsularis) Plant in Different Developmental Stages for Effective Downstream Molecular Analysis

Content Provider	Springer Nature Link
Author	Samanta, Pradipta Sadhukhan, Sanjoy Das, Subrata Joshi, Alpana Sen, Soumitra K. Basu, Asitava
Copyright Year	2011
Abstract	Jute (Corchorus capsularis), as a natural fibre producing plant species, ranks next to cotton only. Today, biotechnological approach has been considered as most accepted means for any genetic improvement of plant species. However, genetic control of the fibre development in jute has not yet been explored sufficiently for desired genetic improvement. One of the major impediments in exploring the genetic architecture in this crop at molecular level is the availability of good quality RNA from field-grown plant tissues mostly due to the presence of high amount of mucilage and phenolics. Development of a suitable RNA isolation method is becoming essential for deciphering developmental stage-specific gene expression pattern related to fibre formation in this crop species. A combination of modified hot borate buffer followed by isopycnic centrifugation (termed as HBIC) was adopted and found to be the best isolation method yielding sufficient quantity (~350–500 μg/gm fresh tissue) and good quality (A$_{260/280}$ ratio 1.88 to 1.91) RNA depending on the developmental stage of stem tissue from field-grown jute plant. The poly A$^{+}$ RNA purified from total RNA isolated by the present method was found amenable to efficient RT-PCR and cDNA library construction. The present development of RNA isolation was found to be appropriate for gene expression analysis related to fibre formation in this economically important jute plant in near future.
Starting Page	109
Ending Page	115
Page Count	7
File Format	PDF
ISSN	10736085
Journal	Molecular Biotechnology
Volume Number	49
Issue Number	2
e-ISSN	15590305
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-02-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Corchorus capsularis Stem tissues RNA extraction RT-PCR cDNA library Protein Science Human Genetics Biochemistry Biological Techniques Biotechnology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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