NDLI: Reference Gene Selection for Quantitative Real-Time PCR in Chrysanthemum Subjected to Biotic and Abiotic Stress

Content Provider	Springer Nature Link
Author	Gu, Chunsun Chen, Sumei Liu, Zhaolei Shan, Hong Luo, Huolin Guan, Zhiyong Chen, Fadi
Copyright Year	2011
Abstract	Quantitative real-time PCR (RT-qPCR) is a reliable method for assessing gene expression, provided that suitable reference genes are included to normalize the data. The stability of expression of eight potential reference genes, namely, tubulin (alpha-2,4 tubulin), actin, EF1α (elongation factor 1α), UBC (ubiquitin C), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), psaA (photosynthesis-related plastid gene representing photosystem I), PP2Acs (catalytic subunit of protein phosphatase 2A), and PGK (phosphoglycerate kinase), was assessed in chrysanthemum plants subjected to aphid infestation, heat stress or waterlogging stress using geNorm software. The widely used reference gene EF1α performed well for aphid infested plants but poorly for waterlogged ones. The catalytic subunit of protein phosphatase 2A (PP2Acs) was the best performing one during heat and waterlogging stress, but was the worst during aphid infestation. The commonly used reference gene actin was generally the least stable of the set. No single gene was suitable for normalization on its own. The choice of reference gene(s) is an important factor in gene expression studies based on RT-qPCR.
Starting Page	192
Ending Page	197
Page Count	6
File Format	PDF
ISSN	10736085
Journal	Molecular Biotechnology
Volume Number	49
Issue Number	2
e-ISSN	15590305
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-03-17
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Reference genes Quantitative real-time PCR Chrysanthemum Normalization Protein Science Human Genetics Biochemistry Biological Techniques Biotechnology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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