NDLI: Galanin Expression in the Mouse Major Pelvic Ganglia During Explant Culture and Following Cavernous Nerve Transection

Content Provider	Springer Nature Link
Author	Girard, Beatrice M. Galli, Jonathan R. Vizzard, Margaret A. Parsons, Rodney L.
Copyright Year	2012
Abstract	Autonomic neurons commonly respond to injury/axotomy with an increased expression of neuropeptides including galanin and pituitary adenylyl cyclase-activating polypeptide (PACAP). The increased peptide expression may enhance neuronal survival and axonal regeneration. Using quantitative (Q) PCR and immunocytochemistry, the present study tested whether galanin expression increased in male mouse major pelvic ganglia (MPG) neurons in response to injury. Galanin transcript expression increased significantly in MPG neurons following 72 h in explant culture and 72 h after unilateral transection of the cavernous nerve. Under both conditions, the increase in galanin transcript levels was greater than the increase in PACAP transcript levels. In control MPG, galanin-IR nerve fibers formed pericellular arrangements around MPG neurons although few galanin-IR cells were evident and many of the galanin-IR cells may be small intensely fluorescent (SIF) cells. In 3-day-cultured MPGs, many more galanin-IR cells and nerve fibers were noted. The increased galanin expression was most apparent in neurons that were also immunoreactive for neuronal nitric oxide synthase, rather than tyrosine hydroxylase. Some explant-cultured MPG neurons exhibited immunoreactivity to galanin and PACAP. As reported previously for PACAP, there is an injury-induced increase in MPG galanin expression, which occurs preferentially in the parasympathetic postganglionic neurons.
Starting Page	713
Ending Page	720
Page Count	8
File Format	PDF
ISSN	08958696
Journal	Journal of Molecular Neuroscience
Volume Number	48
Issue Number	3
e-ISSN	15591166
Language	English
Publisher	Humana Press Inc
Publisher Date	2012-05-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Autonomic neurons Injury response Parasympathetic neurons Immunocytochemistry Quantitative PCR Neurosciences Neurochemistry Neurology Proteomics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Medicine Cellular and Molecular Neuroscience

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