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| Content Provider | Springer Nature Link |
|---|---|
| Author | Pasini, Alice Bonafè, Francesca Govoni, Marco Guarnieri, Carlo Morselli, Paolo G. Sharma, Hari S. Caldarera, Claudio M. Muscari, Claudio Giorda, Emanuele |
| Copyright Year | 2013 |
| Abstract | Adipose-derived stem cells (ADSCs) are stromal mesenchymal stem cells isolated from lipoaspirates, and they display a broad potential to differentiate toward different lineages. The role of epigenetics in regulating the expression of their lineage-specific genes is under evaluation, however till date virtually nothing is known about the relative significance of cardiac-specific transcription factor genes in human ADSCs. The aim of this study was to investigate DNA promoter methylation and relevant histone modifications involving MEF-2C, GATA-4, and Nkx2.5 in native human ADSCs. CpG sites at the transcription start in their promoters were found unmethylated using methylation-specific PCR. Chromatin immunoprecipitation assay showed low levels of total acetylated H3 histone (acH3) and high levels of trimethylated lysine 27 in H3 histone (H3K27me3) which were associated with both GATA-4 and Nkx2.5 promoters, indicating their transcriptional repressive chromatin arrangement. On the other hand, the opposite was apparent for MEF-2C promoter. Accordingly, MEF-2C—but not GATA-4 and Nkx2.5—transcripts were evidenced in native human ADSCs. These results suggest that the chromatin arrangement of these early cardiac regulatory genes could be explored as a level of intervention to address the differentiation of human ADSCs toward the cardiac lineage. |
| Starting Page | 255 |
| Ending Page | 262 |
| Page Count | 8 |
| File Format | |
| ISSN | 10859195 |
| Journal | Cell Biochemistry and Biophysics |
| Volume Number | 67 |
| Issue Number | 2 |
| e-ISSN | 15590283 |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2013-04-27 |
| Publisher Place | Boston |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | DNA methylation Histone modifications Chromatin Epigenetics Adipose-derived stem cells Early cardiac regulatory genes Biochemistry Pharmacology/Toxicology Biotechnology Cell Biology Biophysics and Biological Physics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine Biochemistry Biophysics |
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