NDLI: Cation Modulation of Hemoglobin Interaction with Sodium n-Dodecyl Sulfate (SDS). III: Calcium Interaction with R- and Mixed Spin States of Hemoglobin S at pH 5.0: The Musical Chair Paradox

Content Provider	Springer Nature Link
Author	Nwamba, Charles O. Chilaka, Ferdinand C. Moosavi Movahedi, Ali Akbar
Copyright Year	2013
Abstract	We investigate the interaction of Ca$^{2+}$ (0–500 µM) and a membrane mimic (0.60 mM SDS) with both the R- and mixed spin states hemoglobin S (HbS) as a function of time. These interactions were carried out at pH 5.0. We aim at ascertaining if there is or are differences in the UV–Visible spectra of such interactions to account for the dynamics of calcium ion concentrations [Ca$^{2+}$] in initiating structures which may ultimately suggest HbS polymerization and or resistance to Plasmodium attack. From our results, we conclude that (a) simultaneous interaction of 40 µM Ca$^{2+}$ and 0.60 mM SDS with the R state protein would promote structural formations that can “lock up” the protein for nucleation on the membranes and or become cytotoxic to the parasite; (b) simultaneous R state HbS-SDS or R state HbS-Ca$^{2+}$ would lead to enhanced hemin formation and less deoxyHb species. This condition is unlikely to precipitate polymerization in the HbS but the resulting hemin would poison the parasite; (c) the mixed spin state HbS-SDS and 40 µM Ca$^{2+}$ interaction yields more toxic products to that of the interaction of the mixed spin HbS-SDS with 500 µM Ca$^{2+}$ thus suggesting why the 40 µM Ca$^{2+}$ is important in parasite Hb proteolysis; and (d) pronounced structural changes on interaction with SDS and Ca$^{2+}$ are more in the R state to the mixed spin state.
Starting Page	547
Ending Page	555
Page Count	9
File Format	PDF
ISSN	10859195
Journal	Cell Biochemistry and Biophysics
Volume Number	67
Issue Number	2
e-ISSN	15590283
Language	English
Publisher	Springer US
Publisher Date	2013-03-03
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Conformational plasticity Hemoglobin Membrane Mixed spin state Plasmodium Sickle cell disease (SCD) Sodium n-dodecyl sulfate (SDS) Biochemistry Pharmacology/Toxicology Biotechnology Cell Biology Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Biochemistry Biophysics

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