NDLI: Characterizing Cell–Cell Interactions Induced Spatial Organization of Cell Phenotypes: Application to Density-Dependent Protein Nucleocytoplasmic Distribution

Content Provider	Springer Nature Link
Author	Han, Fujun Zhang, Biliang
Copyright Year	2012
Abstract	Cell–cell interactions play an important role in spatial organization (pattern formation) during the development of multicellular organisms. An understanding of these biological roles requires identifying cell phenotypes that are regulated by cell–cell interactions and characterizing the spatial organizations of the phenotypes. However, conventional methods for assaying cell–cell interactions are mainly applicable at a cell population level. These measures are incapable of elucidating the spatial organizations of the phenotypes, resulting in an incomplete view of cell–cell interactions. To overcome this issue, we developed an automated image-based method to investigate cell–cell interactions based on spatial localizations of cells. We demonstrated this method in cultured cells using cell density-dependent nucleocytoplasmic distribution of β-catenin and aryl hydrocarbon receptor as the phenotype. This novel method was validated by comparing with a conventional population-based method, and proved to be more sensitive and reliable. The application of the method characterized how the phenotypes were spatially organized in a population of cultured cells. We further showed that the spatial organization was governed by cell density and was protein-specific. This automated method is very simple, and will be applicable to study cell–cell interactions in different systems from prokaryotic colonies to multicellular organisms. We envision that the ability to extract and interpret how cell–cell interactions determine the spatial organization of a cell phenotype will provide new insights into biology that may be missed by traditional population-averaged studies.
Starting Page	163
Ending Page	172
Page Count	10
File Format	PDF
ISSN	10859195
Journal	Cell Biochemistry and Biophysics
Volume Number	65
Issue Number	2
e-ISSN	15590283
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-08-23
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aryl hydrocarbon receptor Cell–cell interactions Cell–cell communication Cell density Cell phenotype Nucleocytoplasmic distribution Image analysis Spatial organization β-Catenin Biochemistry Pharmacology/Toxicology Biotechnology Cell Biology Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Medicine Biochemistry Biophysics

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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