NDLI: Impact of LPS-Induced Cardiomyoblast Cell Apoptosis Inhibited by Earthworm Extracts

Content Provider	Springer Nature Link
Author	Li, Ping Chun Tien, Yun Chen Day, Cecilia Hsuan Pai, Peiying Kuo, Wei Wen Chen, Tung Sheng Kuo, Chia Hua Tsai, Chang Hai Ju, Da Tong Huang, Chih Yang
Copyright Year	2014
Abstract	Dilong is an earthworm extract with a dense nutritional content, widely used in Chinese herbal medicine to remove stasis and stimulate wound healing. Earthworm extracts are traditionally used by indigenous people throughout the world. How this Dilong inhibits Lipopolysaccharide (LPS)-induced cardiomyoblast cell apoptosis is still unclear. This study investigates the Dilong extract effect on LPS-induced apoptosis in H9c2 cardiomyoblast cells. LPS (1 μg/ml) administration for 24 h induced apoptosis in H9c2 cells. Cell apoptosis was detected using MTT, LDH, TUNEL assay and JC-1 staining. Western blot analysis was used to detect pro-apoptotic and anti-apoptotic proteins. Dilong extract totally blocked the LPS impact, leading to the activation of anti-apoptotic proteins, Bcl-2 and Bcl-xL, stabilized the mitochondria membrane and down-regulated the extrinsic and intrinsic pro-apoptotic proteins, TNF-α, active caspase-8, t-Bid, Bax, active caspase-9 and active caspase-3. Dilong could potentially serve as a cardio protective agent against LPS-induced H9c2 cardiomyoblast cell apoptosis.
Starting Page	172
Ending Page	179
Page Count	8
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	15
Issue Number	2
e-ISSN	15590259
Language	English
Publisher	Springer US
Publisher Date	2014-09-24
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	H9c2 cardiomyoblast cells Apoptosis Lipopolysaccharide Dilong Sepsis Pharmacology/Toxicology Cardiology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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