NDLI: Gene expression profiles of mouse aorta and cultured vascular smooth muscle cells differ widely, yet show common responses to dioxin exposure

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Gene expression profiles of mouse aorta and cultured vascular smooth muscle cells differ widely, yet show common responses to dioxin exposure

Cardiovascular Toxicology : Volume 4, Issue 3, September 2004

Cardiovascular Toxicology : Volume 4, Issue 2, June 2004

Cardiovascular Toxicology : Volume 4, Issue 1, March 2004

Cardiovascular Toxicology : Volume 3

Cardiovascular Toxicology : Volume 2

Cardiovascular Toxicology : Volume 1

Gene expression profiles of mouse aorta and cultured vascular smooth muscle cells differ widely, yet show common responses to dioxin exposure

Content Provider	Springer Nature Link
Author	Puga, Alvaro Sartor, Maureen A. Huang, Ming ya Kerzee, J. Kevin Wei, Yu Dan Tomlinson, Craig R. Baxter, C. Stuart Medvedovic, Mario
Copyright Year	2004
Abstract	Exposure to environmental toxicants may play a role in the onset and progression of cardiovascular disease. Many environmental agents, such as dioxin, are risk factors for atherosclerosis because they may exacerbate an underlying disease by altering gene expression patterns. Expression profiling of vascular tissues allows the simutaneous analysis of thousands of genes and may provide predictive information particularly useful in early disease stages. Often, however, in vivo experiments are unfeasible for material or ethical reasons, and data from cultured cells must be used instead, even though it may not be known whether cultured cells and live tissues share common global responses to the same toxicant. In a search for gene responsive to dioxin exposure, we used oligonucleotide microarrays with DNA sequences from 13,433 genes to compare global gene expression profiles of C57BL/6 mice aortas with cultured vascular smooth muscle cells (vSMCs) of the same mice. Aorta segments and vSMCs differed in the expression of more than 4500 genes, many showing expression differences greater than 1000-fold. Integration of microarray data into Gene Ontology Project annotations showed that many of the genes differentially expressed belonged to the same biological process or metabolic pathway. Notwithstanding these results, a subset of 35 genes responded in the same fashion to dioxin exposure in both systems. Genes in this subset encoded phase I and phase II detoxification enzymes, signal transduction kinases and phosphatases, and proteins involved in DNA repair and the cell cycle. We conclude that vSMCS may be useful aorta surrogates to study early gene expression responses to dioxin exposure, provided that analyses focus on this subset of genes.
Starting Page	385
Ending Page	403
Page Count	19
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	4
Issue Number	4
e-ISSN	15590259
Language	English
Publisher	Humana Press
Publisher Date	2004-01-01
Publisher Place	Totowa
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vascular smooth muscle cells TCDD atherosclerosis gene ontology Gene Microarray Pathway Profiler oligonucleotide microarrays Pharmacology/Toxicology Cardiology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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