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Reduction of protein synthesis and statin-induced cardiomyocyte cell death

Content Provider	Springer Nature Link
Author	Rabkin, Simon W. Lodha, Parth Kong, Jennifer Y.
Copyright Year	2007
Abstract	The objective of this study was to determine whether an HMG Co A reductase inhibitor (statin) reduces protein synthesis in cardiomyocytes and whether this action maybe an underlying mechanism for statin-induced cell death. Cardiomyocytes from embryonic chick heart were maintained in culture. Cells exposed to lovastatin for 4 h showed a concentration dependent reduction in protein synthesis as assessed by [$^{3}$H] leucine incorporation and [$^{35}$S] methionine incorporation. Compared to control, lovastatin 100 μM, which produced a 25% increase in cell death, induced a three-fold reduction in methionine incorporation. [$^{35}$S] methionine autoradiography showed little (new) protein synthesis at concentrations of lovastatin of 70 μM or higher; an effect that was not limited to specific proteins. Cardiomyocytes treated with lovastatin showed morphologic changes in the nucleoli consistent with insufficient protein synthesis. These cardiomyocytes manifested cell death under conditions of reduced protein synthesis. Interruption of protein synthesis with cycloheximide, a ribosomal RNA transcription inhibitor or reduction in protein substrate availability by lowering the media concentration of fetal calf serum was associated with a concentration-dependent reductions in cell viability. Importantly, stimulation of protein synthesis by higher concentrations of fetal calf serum limited lovastatin-induced cell death. These data suggest that statin-induced inhibition of protein synthesis is an underlying mechanism for statin-induced cell death.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	15307905
Journal	Cardiovascular Toxicology
Volume Number	7
Issue Number	1
e-ISSN	15590259
Language	English
Publisher	Humana Press Inc
Publisher Date	2007-04-19
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Lovastatin Cardiomyocyte Cell death Protein synthesis heart Cardiology Pharmacology/Toxicology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Toxicology Cardiology and Cardiovascular Medicine

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