NDLI: Synthesis of trace element Si and Sr codoping hydroxyapatite with non-cytotoxicity and enhanced cell proliferation and differentiation

Content Provider	Springer Nature Link
Author	Gao, Jianyong Wang, Ming Shi, Chao Wang, Liping Wang, Dalin Zhu, Yingchun
Copyright Year	2016
Abstract	The main inorganic minerals in natural bones are non-stoichiometric hydroxyapatite (HA, Ca$_{10}$[PO$_{4}$]$_{6}$[OH]$_{2}$) doped with various trace elements, which may possess important biochemical effects. To investigate the functions of Sr and Si elements in human hard tissues, non-doped HA, trace Si doped HA, Si and Sr codoped HA with the concentration of natural bones are synthesized by hydrothermal method in this study. The samples are characterized by powder X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscope (SEM). The biological activities are evaluated via cytotoxicity study, adhesion and proliferation of osteoblast measurement, and alkaline phosphatase (ALP) assay. All the synthesized materials are HA phase, which have hierarchical structures with oriented HA nanorods assembled into the platy particles. These materials are non-cytotoxic against L929 cells line even at 400 μg/ml powder suspension. The results clearly indicate that the proliferation of L929 cells increases with trace elements doping from trace Si-HA to Si + Sr-HA. The adhesion and proliferation of osteoblast measurement illustrates that proliferation of osteoblasts advances about 1.3 times for Si-HA and about 1.8 times for Si + Sr-HA compared with undoped HA. In general, Si-HA with trace Si element shows enhanced cell differentiation, and Si + Sr-HA dual-doped with Si and Sr elements presents increased biological activity compared with Si-HA.
Starting Page	208
Ending Page	217
Page Count	10
File Format	PDF
ISSN	01634984
Journal	Biological Trace Element Research
Volume Number	174
Issue Number	1
e-ISSN	15590720
Language	English
Publisher	Springer US
Publisher Date	2016-04-13
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hydroxyapatite Codoping Trace element Si Sr Cell proliferation and differentiation Biochemistry Biotechnology Nutrition Oncology
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Endocrinology, Diabetes and Metabolism Clinical Biochemistry Biochemistry Inorganic Chemistry

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