NDLI: In Vivo Evaluation of Cerebral Transplantation of Resovist-Labeled Bone Marrow Stromal Cells in Parkinson’s Disease Rats Using Magnetic Resonance Imaging

Content Provider	Springer Nature Link
Author	Guo, Jing Shen, Jun Kang Wang, Lan Xiao, Li Zhang, Rong Jun Luo, Wei Feng Gong, Zhi Gang Sun, Jing Xu, Han Sirois, Pierre Li, Kai
Copyright Year	2010
Abstract	The effectiveness of Resovist-labeled bone marrow stem cells (BMSCs) was evaluated in vivo following their cerebral transplantation in a model of Parkinson’s disease (PD) in rats using MRI, and the MRI findings were further compared with the behavior and histopathological manifestations of these rats. Forty PD rats were randomly assigned into five groups according to the cell doses injected into the rat brain site: control group (normal saline injection) and groups injected with 1 × 10$^{5}$, 1.5 × 10$^{5}$, 2 × 10$^{5}$, and 2.5 × 10$^{5}$ BMSCs. Gradient echo T2-weighted images were obtained immediately after cell transplantation and repeatedly taken 1, 4, 8, and 12 week(s) after cell transplantation. The rotational behavior of the animals was observed before and 1, 4, and 8 week(s) after transplantation. The rats were killed after the last MRI scanning, the brain tissues were analyzed by histopathology techniques, and RNAs were extracted for the expression analysis of selected genes using RT-PCR. One week following cell transplantation, all injected sites showed well-defined hypointense areas on MR images, with the most significant effect observed in rats injected with 2 × 10$^{5}$ BMSCs. These MR findings in PD rats lasted up to 12 weeks. The effectiveness of BMSC transplantation revealed by MRI was well confirmed by the behavioral and histopathological observations as well as indirectly supported by gene expression analyses. With the use of SPIO labeling, MRI techniques provided a dynamic evaluation of the spatial and temporal changes following cell transplantation and allowed the association analysis among the imaging, functions, and gene expression analysis in rats. These data also suggest the therapeutic potential of transplanted BMSCs. It is reasonable to speculate that the use of MRI in in vivo evaluation of the effect and fate of transplanted cells in various disease models will be beneficial to developing new strategies of cell-based gene therapy.
Starting Page	636
Ending Page	648
Page Count	13
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	163
Issue Number	5
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-09-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	BMSCs Cell transplantation MRI PD Resovist SPIO Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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