NDLI: Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes

Content Provider	Springer Nature Link
Author	Brumm, Phillip Mead, David Boyum, Julie Drinkwater, Colleen Gowda, Krishne Stevenson, David Weimer, Paul
Copyright Year	2010
Abstract	Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of Fisuc was completed by the DOE Joint Genome Institute in late 2009. Using new expression tools developed at Lucigen and C5-6 Technologies and a multi-substrate screen, 5,760 random shotgun expression clones were screened for biomass-degrading enzymes, representing 2× genome expression coverage. From the screen, 169 positive hits were recorded and 33 were unambiguously identified by sequence analysis of the inserts as belonging to CAZy family genes. Eliminating duplicates, 24 unique CAZy genes were found by functional screening. Several previously uncharacterized enzymes were discovered using this approach and a number of potentially mis-annotated enzymes were functionally characterized. To complement this approach, a high-throughput system was developed to clone and express all the annotated glycosyl hydrolases and carbohydrate esterases in the genome. Using this method, six previously described and five novel CAZy enzymes were cloned, expressed, and purified in milligram quantities.
Starting Page	649
Ending Page	657
Page Count	9
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	163
Issue Number	5
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2010-08-28
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fibrobacter succinogenes Biomass Genome screening Cellulase Hemicellulase Rumen Screening Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Erratum: Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes

Erratum: Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes

Functional annotation of Fibrobacter succinogenes S85 carbohydrate active enzymes.

Mining Dictyoglomus turgidum for Enzymatically Active Carbohydrases

A global analysis of gene expression in Fibrobacter succinogenes S85 grown on cellulose and soluble sugars at different growth rates

Utility of enzymes from Fibrobacter succinogenes and Prevotella ruminicola as detergent additives

Application of a Data Reconciliation Method to the Stoichiometric Analysis of Fibrobacter succinogenes Growth

Cellulose digestion and cellulase regulation and distribution in Fibrobacter succinogenes subsp. succinogenes S85.

Production of oligosaccharides and cellobionic acid by Fibrobacter succinogenes S85 growing on sugars, cellulose and wheat straw

Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes

Similar Documents

Erratum: Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes

Erratum: Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes

Functional annotation of Fibrobacter succinogenes S85 carbohydrate active enzymes.

Mining Dictyoglomus turgidum for Enzymatically Active Carbohydrases

A global analysis of gene expression in Fibrobacter succinogenes S85 grown on cellulose and soluble sugars at different growth rates

Utility of enzymes from Fibrobacter succinogenes and Prevotella ruminicola as detergent additives

Application of a Data Reconciliation Method to the Stoichiometric Analysis of Fibrobacter succinogenes Growth

Cellulose digestion and cellulase regulation and distribution in Fibrobacter succinogenes subsp. succinogenes S85.

Production of oligosaccharides and cellobionic acid by Fibrobacter succinogenes S85 growing on sugars, cellulose and wheat straw

Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes