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| Content Provider | Springer Nature Link |
|---|---|
| Author | Safwani, Wan Kamarul Zaman Wan Makpol, Suzana Sathapan, Somasundaram Chua, Kien Hui |
| Copyright Year | 2012 |
| Abstract | Human adipose-derived stem cells (ASCs) have generated a great deal of excitement in regenerative medicine. However, their safety and efficacy issue remain a major concern especially after long-term in vitro expansion. The aim of this study was to investigate the fundamental changes of ASCs in long-term culture by studying the morphological feature, growth kinetic, surface marker expressions, expression level of the senescence-associated genes, cell cycle distribution and ß-galactosidase activity. Human ASCs were harvested from lipoaspirate obtained from 6 patients. All the parameters mentioned above were measured at P5, P10, P15 and P20. Data were subjected to one-way analysis of variance with a Tukey post hoc test to determine significance difference (P < 0.05). The data showed that growth of ASCs reduced in long-term culture and the ß-galactosidase activity was significantly increased at later passage (P20). The morphology of ASCs in long-term culture showed the manifestation of senescent feature at P15 and P20. Significant alteration in the senescence-associated genes expression levels was observed in MMP1, p21, Rb and Cyclin D1 at P15 and P20. Significant increase in CD45 and HLA DR DQ DP surface marker was observed at P20. While cell cycle analysis showed significant decrease in percentage of ASCs at S and G2/M phase at later passage (P15). Our data showed ASCs cultured beyond P10 favours the senescence pathway and its clinical usage in cell-based therapy may be limited. |
| Starting Page | 2101 |
| Ending Page | 2113 |
| Page Count | 13 |
| File Format | |
| ISSN | 02732289 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume Number | 166 |
| Issue Number | 8 |
| e-ISSN | 15590291 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-03-07 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Adipose-derived stem cells Long-term culture Senescence Cell-based therapy Regenerative medicine Biotechnology Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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