NDLI: Angiotensin I-Converting Enzyme Inhibitory Proteins and Peptides from the Rhizomes of Zingiberaceae Plants

Content Provider	Springer Nature Link
Author	Yodjun, Maneerat Karnchanatat, Aphichart Sangvanich, Polkit
Copyright Year	2012
Abstract	Ammonium sulphate cut protein extracts, and their pepsin hydrolysates, from the rhizomes of 15 plants in the Zingiberaceae family were screened for their in vitro angiotensin I-converting enzyme inhibitory (ACEI) activity. The protein extract from Zingiber ottensii had the highest ACEI activity (IC$_{50}$ of 7.30 × 10$^{−7}$ mg protein/mL) and was enriched for by SP Sepharose chromatography with five NaCl step gradients 0, 0.25, 0.50, 0.75 and 1 M NaCl collecting the corresponding five fractions. The highest ACEI activity was found in the F75 fraction, which appeared to contain a single 20.7-kDa protein, suggesting enrichment to or near to homogeneity. The ACEI activity of the F75 fraction was moderately thermostable (−20–60 °C), showed >80% activity across a broad pH range of 4–12 (optimal at pH 4–5) and appeared as a competitive inhibitor of ACE (K $_{i}$ of 9.1 × 10$^{−5}$ mg protein/mL). For the pepsin hydrolysates, that from Zingiber cassumunar revealed the highest ACEI activity (IC$_{50}$ of 0.38 ± 0.012 mg/mL), was enriched to a single active hexapeptide by RP-HPLC with a strong ACEI activity (IC$_{50}$ of 0.011 ± 0.012 mg/mL) and acted as a competitive inhibitor of ACE (K $_{i}$ of 1.25 × 10$^{−6}$ mg protein/mL).
Starting Page	2037
Ending Page	2050
Page Count	14
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	166
Issue Number	8
e-ISSN	15590291
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-03-07
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Angiotensin I-converting enzyme Proteins Peptides Zingiberaceae plant Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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