NDLI: Induction of a High-Yield Lovastatin Mutant of Aspergillus terreus by $^{12}$C$^{6+}$ Heavy-Ion Beam Irradiation and the Influence of Culture Conditions on Lovastatin Production Under Submerged Fermentation

Content Provider	Springer Nature Link
Author	Li, Shi Weng Li, Mei Song, Hong Ping Feng, Jia Li Tai, Xi Sheng
Copyright Year	2011
Abstract	Heavy-ion beams, possessing a wide mutation spectrum and increased mutation frequency, have been used effectively as a breeding method. In this study, the heavy-ion beams generated by the Heavy-Ion Research Facility in Lanzhou were used to mutagenize Aspergillus terreus CA99 for screening high-yield lovastatin strains. Furthermore, the main growth conditions as well as the influences of carbon and nitrogen sources on the growth and the lovastatin production of the mutant and the original strains were investigated comparatively. The spores of A. terreus CA99 were irradiated by 15, 20, 25, and 30 Gy of 80 MeV/u $^{12}$C$^{6+}$ heavy-ion beams. Based on the lovastatin contents in the fermentation broth, a strain designated as A. terreus Z15-7 has been selected from the clone irradiated by the heavy-ion beam. When compared with the original strain, the content of lovastatin in the fermentation broth of A. terreus Z15-7 increased 4-fold. Moreover, A. terreus Z15-7 efficiently used the carbon and nitrogen sources for the growth and production of lovastatin when compared to the original strain. The maximum yield of lovastatin, 916.7 μg/ml, was obtained as A. terreus Z15-7 was submerged cultured in the chemically defined medium supplemented with 3% glycerol as a carbon source, 1% corn meal as an organic nitrogen source, and 0.2% sodium nitrate as an inorganic nitrogen source at 30 °C in the shake flask. The result shows that heavy-ion beam irradiation is an effective method for the mutation breeding of lovastatin production of A. terreus.
Starting Page	913
Ending Page	925
Page Count	13
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	165
Issue Number	3-4
e-ISSN	15590291
Language	English
Publisher	Humana Press Inc
Publisher Date	2011-06-28
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Aspergillus terreus Heavy-ion beam Mutation breeding Lovastatin Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Induction of a high-yield lovastatin mutant of Aspergillus terreus by ¹²C6âº heavy-ion beam irradiation and the influence of culture conditions on lovastatin production under submerged fermentation.

Enhancement of Lovastatin Production by Supplementing Polyketide Antibiotics to the Submerged Culture of Aspergillus terreus

Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus ( KM 017963 ) under Solid State Fermentation

Utilisation of vegetable oils in the production of lovastatin by Aspergillus terreus ATCC 20542 in submerged cultivation

Evaluating the outcomes of submerged co-cultivation: production of lovastatin and other secondary metabolites by Aspergillus terreus in fungal co-cultures.

Effects of ion beam irradiation on mutation induction in rice.

Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation

Lovastatin inhibits its own synthesis in Aspergillus terreus

Optimization of lovastatin production in solid state fermentation by Aspergillus terreus

Induction of a High-Yield Lovastatin Mutant of Aspergillus terreus by $^{12}$C$^{6+}$ Heavy-Ion Beam Irradiation and the Influence of Culture Conditions on Lovastatin Production Under Submerged Fermentation

Similar Documents

Induction of a high-yield lovastatin mutant of Aspergillus terreus by ¹²C6âº heavy-ion beam irradiation and the influence of culture conditions on lovastatin production under submerged fermentation.

Enhancement of Lovastatin Production by Supplementing Polyketide Antibiotics to the Submerged Culture of Aspergillus terreus

Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus ( KM 017963 ) under Solid State Fermentation

Utilisation of vegetable oils in the production of lovastatin by Aspergillus terreus ATCC 20542 in submerged cultivation

Evaluating the outcomes of submerged co-cultivation: production of lovastatin and other secondary metabolites by Aspergillus terreus in fungal co-cultures.

Effects of ion beam irradiation on mutation induction in rice.

Lovastatin biosynthetic genes of Aspergillus terreus are expressed differentially in solid-state and in liquid submerged fermentation

Lovastatin inhibits its own synthesis in Aspergillus terreus

Optimization of lovastatin production in solid state fermentation by Aspergillus terreus

Induction of a High-Yield Lovastatin Mutant of Aspergillus terreus by $^{12}$C$^{6+}$ Heavy-Ion Beam Irradiation and the Influence of Culture Conditions on Lovastatin Production Under Submerged Fermentation

Induction of a high-yield lovastatin mutant of Aspergillus terreus by ¹²C6âº heavy-ion beam irradiation and the influence of culture conditions on lovastatin production under submerged fermentation.