NDLI: Production of Potential Vaccine Against Dermatobia hominis for Cattle

Content Provider	Springer Nature Link
Author	Fernandes, Nelson L. M. Zanata, Silvio M. Rönnau, Milton Soccol, Carlos R. Pandey, Ashok Thomaz Soccol, Vanete
Copyright Year	2012
Abstract	The present study aimed to detect and characterize antigenic proteins and to assess their activity as preventive vaccines against dermatobiosis. Polyclonal antibodies were produced against three larval instars (L$_{1}$, L$_{2}$, L$_{3}$), and their antigenic proteins were assessed for reactivity. Polyclonal antibodies produced in animals immunized with extracts were analyzed, and L$_{3}$-derived antibodies showed proteins with better antigenic responses. The study of reactivity using immunodetection showed that the 50-kDa protein had the highest antigenicity. This protein was purified and subjected to mass spectrometry, and the sequences obtained were compared with those in the databases available. No similarities were found with existing sequences. Subsequently, large quantities of purified protein were used to immunize cattle. Vaccine effectiveness was evaluated by comparing the number of cutaneous nodules formed in the control group and immunized animals. The antigen produced proved a promising candidate for vaccine production, with 90.67 % efficacy. Immunohistochemistry of antigen–antibody reaction in larval sections showed epitopes all over larval tissues.
Starting Page	412
Ending Page	424
Page Count	13
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	167
Issue Number	3
e-ISSN	15590291
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-04
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dermatobia hominis Antigen selection Immunohistochemistry Cattle Vaccine Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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Production of potential vaccine against dermatobia hominis for cattle

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