NDLI: Antimicrobial Potential of Callistemon lanceolatus Seed Extract and its Statistical Optimization

Content Provider	Springer Nature Link
Author	Arora, Daljit Singh Nim, Lovedeep Kaur, Harpreet
Copyright Year	2016
Abstract	Plants have always been eminent source of medicinal products. Screening of the aqueous seeds extract of Callistemon lanceolatus (bottle brush) revealed its broad spectrum antimicrobial potential with an inhibition zone ranging from 13 to 28 mm against various pathogenic microorganisms. While optimizing the different parameters the antimicrobial activity was better expressed at 15 % concentration, prepared by extracting the material at 60 °C for 20 min. The extract was filtered through muslin cloth and gave best results at its natural pH. Statistical optimization by Response surface methodology enhanced the antimicrobial activity up to 1.6-fold. Minimum inhibitory concentration values of the aqueous extract of seeds of C. lanceolatus against different organisms ranged from 1–5 mg/ml. The viable cell count studies indicated a bactericidal effect against most of the pathogens. The aqueous extract was found to be relatively thermostable at 100 °C. When treated for shelf life at ambient conditions and refrigeration temperature (2–8 °C), the latter only showed a 28 % loss in antimicrobial activity. The aqueous extract was found to be biosafe when evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide reagent (MTT toxicity) assay and Ames mutagenicity assay.
Starting Page	289
Ending Page	305
Page Count	17
File Format	PDF
ISSN	02732289
Journal	Applied Biochemistry and Biotechnology
Volume Number	180
Issue Number	2
e-ISSN	15590291
Language	English
Publisher	Springer US
Publisher Date	2016-05-14
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Antimicrobial Callistemon lanceolatus MIC Biosafety Biotechnology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology

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