NDLI: Identification and properties of bupivacaine-sensitive potassium currents in cultured hippocampal neurons

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Identification and properties of bupivacaine-sensitive potassium currents in cultured hippocampal neurons

Content Provider	Springer Nature Link
Author	Konakov, M. V. Berezhv, A. V. Teplov, I. Yu. Levin, S. G. Godukhin, O. V.
Copyright Year	2015
Abstract	The aim of this study was to identify and study the properties of a bupivacaine-sensitive potassium current in the plasma membrane of cultured neurons of the rat brain hippocampus. A neuroglial cell culture used in the work was obtained from the brain hippocampus of Wistar rats aged 1–3 days. The neuronal activity was recorded after 10–14 days of culturing. Bupivacaine-sensitive potassium current and the membrane potential (MP) of the analyzed neurons were recorded by the perforated patch-clamp technique. Neurons were identified visually by their morphology, as well as by the pattern of their electrical activity upon depolarizing shifts of the MP. The identified potassium currents were blocked by the application of local anesthetic bupivacaine and non-selective blocker of potassium channels barium and had a reversal potential close to the equilibrium potential for K$^{+}$. Effects of bupivacaine were fully prevented by Ba$^{2+}$, while bupivacaine did not completely block the Ba$^{2+}$ effects, suggesting that bupivacaine affected only a fraction of the Ba$^{2+}$-blockable membrane channels. Lowering the extracellular pH from 7.4 to 6.3 resulted in an inhibition of the identified potassium current, similarly to the effect of bupivacaine, whereas an increase in the extracellular pH up to 8.2 led to the current augmentation. Current-voltage characteristics for bupivacaine-sensitive potassium current was obtained; at a hyperpolarizing shift of MP to–120 mV the current amplitude was–33 ± 6 pA. The obtained results testify that the properties of bupivacaine-sensitive potassium current in the 10–14-day cultured hippocampal neurons correspond to those of the background 2P potassium channels of the TASK subfamily.
Starting Page	309
Ending Page	317
Page Count	9
File Format	PDF
ISSN	19907478
Journal	Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
Volume Number	9
Issue Number	4
e-ISSN	19907494
Language	English
Publisher	Pleiades Publishing
Publisher Date	2015-12-23
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	background 2P potassium channels TASK subfamily of 2P potassium channels bupivacaine patch clamp hippocampal neurons potassium currents membrane potential Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Biophysics

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