NDLI: Fragmentation of coding region chromatin of trp-dioxygenase (to) and tyr-aminotransferase (tat) genes in their active and repressed states by micrococcal nuclease

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Fragmentation of coding region chromatin of trp-dioxygenase (to) and tyr-aminotransferase (tat) genes in their active and repressed states by micrococcal nuclease

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Cell and Tissue Biology : Volume 5, Issue 3, June 2011

Cell and Tissue Biology : Volume 5, Issue 2, April 2011

Cell and Tissue Biology : Volume 5, Issue 1, February 2011

Cell and Tissue Biology : Volume 4

Cell and Tissue Biology : Volume 3

Cell and Tissue Biology : Volume 2

Cell and Tissue Biology : Volume 1

Fragmentation of coding region chromatin of trp-dioxygenase (to) and tyr-aminotransferase (tat) genes in their active and repressed states by micrococcal nuclease

Content Provider	Springer Nature Link
Author	Priyatkina, T. N. Pavlova, E. Yu. Voiva, N. E. Deforges, E. V.
Copyright Year	2011
Abstract	The pattern of DNA fragmentation by mirococcal nuclease (MNase) of tyr-aminotransferase (tat) and trp-dioxygenase (to) genes in active (rat liver nuclei) and repressed (rat brain nuclei) states has been examined by the blot-hybridization technique. It was found that, in rat liver nuclei, to and tat coding regions lack nuclease-resistant compact particles (nucleosomes) divided by free DNA segments (internucleosome linkers). Within a wide range of enzyme concentrations, two types of fragments are mainly produced, i.e., approximately full-sized to- and tat-transcriptional units (19 and 11 kbp, respectively) and large (from 1500 bp) segments that are heterogeneous in size. To and tat fragments of both types are preserved in hydrolysates at a limit of the MNase digestion of total chromatin DNA when nuclease breaks occur in nearly all accessible sites of chromatin. This means that these fragments originate from two distinct subsets of transcription units that coexisted in liver nuclei. Some of them do not contain MNase-accessible sites over their entire length, whereas in other transcription units, resistant regions alternate with rare irregularly located MNase-sensitive regions. We presume that the unavailability of DNA to MNase along the whole length of coding regions is inherent to transcriptionally competent to and tat copies not yet transcribed. Transcription generates MNase-sensitive areas that it is possible flank elongating RNA polymerases.
Starting Page	366
Ending Page	377
Page Count	12
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	5
Issue Number	4
e-ISSN	19905203
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2011-08-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	chromatin of the active gene nuclease fragmentation transcription competence nucleosome remodeling Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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Fragmentation of coding region chromatin of trp-dioxygenase (to) and tyr-aminotransferase (tat) genes in their active and repressed states by micrococcal nuclease

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Fragmentation of coding region chromatin of trp-dioxygenase (to) and tyr-aminotransferase (tat) genes in their active and repressed states by micrococcal nuclease