NDLI: Exogenous NADP increases the level of histone H2AX phosphorylation in mouse heart cells after ionizing radiation

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Exogenous NADP increases the level of histone H2AX phosphorylation in mouse heart cells after ionizing radiation

Content Provider	Springer Nature Link
Author	Firsav, D. V. Kropotov, A. V. Mikhailov, V. M.
Copyright Year	2011
Abstract	Phosphorylation of replacement histone H2AX occurs in megabase chromatin domains around DNA double-strand breaks (DSBs), and this modification called γ-H2AX can be used as an effective marker for DSBs repair and DNA damage response. Using Western blotting and immunohistochemistry techniques we have studied here the influence of exogenous nicotinamide adenine dinucleotide phosphate (NADP), which can potentially increase the level of intracellular NAD$^{+}$, on the level of γ-H2AX formation in mouse heart cells after ionizing radiation (IR). We have found that injection of NADP in different doses immediately after IR causes an increased level of γ-H2AX in mouse heart cells 20 min after IR at the dose of 3 Gy compared to control mice after IR exposure. It indicates that there could be a relationship between intracellular NAD$^{+}$ content and DNA damage response in vivo.
Starting Page	383
Ending Page	387
Page Count	5
File Format	PDF
ISSN	1990519X
Journal	Cell and Tissue Biology
Volume Number	5
Issue Number	4
e-ISSN	19905203
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2011-08-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ionizing radiation Histone H2AX phosphorylation NADP DNA damage response Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology

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