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| Content Provider | Springer Nature Link |
|---|---|
| Author | Bogolyubov, D. S. Bogolyubova, I. O. |
| Copyright Year | 2007 |
| Abstract | The spider Araneus diadematus has solitarily developing oocytes. Microinjections of 5-bromouridine 5′-triphosphate into the ooplasm demonstrated that the oocyte nuclei are transcriptionally active. The nucleus contains several extrachromosomal structures of perfectly spherical shape, the so-called “endobodies.” Laser scanning confocal microscopy using an antibody against the splicing factor SC35, a major component of interchromatin granule clusters (IGCs), revealed intense fluorescence of endobodies. Colocalization of SC35 protein with coilin, a marker protein of Cajal bodies (CBs), was also observed within these structures. We suggest that the endobodies of A. diadematus oocytes may have some features of both IGCs and CBs and represent a single nuclear domain. |
| Starting Page | 352 |
| Ending Page | 356 |
| Page Count | 5 |
| File Format | |
| ISSN | 1990519X |
| Journal | Cell and Tissue Biology |
| Volume Number | 1 |
| Issue Number | 4 |
| e-ISSN | 19905203 |
| Language | English |
| Publisher | Nauka/Interperiodica |
| Publisher Date | 2007-01-01 |
| Publisher Place | Moscow |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Arachnida oocyte nucleus nuclear domains Cajal bodies interchromatin granule clusters confocal microscopy Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology |
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